lienlab LABORATOIRE DE BIOGENESE MEMBRANAIRE
UMR 5200 CNRS

Université 
Bordeaux Segalen
Bâtiment A3
INRA
Bordeaux Aquitaine
71 Avenue Edouard Bourlaux
CS 20032
33140 Villenave d'Ornon

 
Tél: 00 33 5 57 12 25 89
Fax: 00 33 5 57 12 26 44

Directeur : Dr Jean-Jacques Bessoule
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Dernière mise à jour : 12 Juillet 2016
Responsable éditorial: Jean-Jacques Bessoule
Responsable Com: Sébastien Mongrand
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[July 3-8, 2016] Jerome, Yijun, Vincent and Fred came back from Goettigen in Germany where they attended the 22th International Symposium on Plant Lipids organized by Ivo Feussner. Jerome gave a talk on the function of CER1 proteins in the biosynthesis of cuticular alkanes in Arabidopsis.

[July 4-7, 2016]
Laetitia and Patrick participated to the annual meeting of the SEB (Society of Experimental Biology) in Brighton, UK on 4 to 7 of July. Patrick gave a talk entitled "Lipid physical properties, membrane dynamics and transport, and lysophospholipid acyltransferases at the ER-Golgi interface in plant cells". Laetitia and Patrick organised a workshop on Plant ER membrane lipids: analysis, composition and functional aspects.

[June 6, 2016] Laetitia contributed to a publication in PNAS on the Fragile X Syndrome pathology. This work supports a model where FMRP (Fragile X Mental Retardation Protein), by controlling the translation of the DiacylGlycerol Kinase kappa (DGKκ), indirectly controls synaptic protein translation and membrane properties by impacting lipid signaling in dendritic spine.
Tabet R., Moutin E., Becker J.A., Heintz D., Fouillen L., Flatter E., Krężel W., Alunni V., Koebel P., Dembélé D., Tassone F., Bardoni B., Mandel J.L., Vitale N., Muller D., Le Merrer J., Moine H. (2016) Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase activity in neurons. Proc Natl Acad Sci U S A, in press

[
May 30, 2016] Camille, Laetitia, Stéphanie, Jérôme and Fred, in collaboration with Owen Rowland (Carleton University, Canada) and Dylan Kosma (University of Reno, USA), published a paper showing that most of the fatty alcohols not covalently linked to the suberin polymer are in the form of alkyl hydroxycinnamates (AHCs), with alkyl caffeates predominating. Such compounds are not restricted to the periderm of mature roots, but are also present in the endodermis of younger roots, where they are not extracted by rapid dipping in chloroform.
 
Delude C., Fouillen L., Bhar P., Cardinal M.-J., Pascal S., Santos P., Kosma D.K., Joubès J., Rowland O. and Domergue F. (2016) Primary fatty alcohols are major components of suberized root tissues of Arabidopsis in the form of alkyl hydroxycinnamates. Plant Physiol., doi:10.1104/pp.16.00834

[May 30, 2016] The ANR projet blanc PANACEA " PlANt plAsma membrane lipid signalisation in biotiC interactions " coordinated by Sébastien in collaboration with three laboratories in Dijon (UMR PME Plante-Microbe-Environnment), Paris (UMR PCMP Physiologie Cellulaire et Moléculaire des Plantes) and Bordeaux (CBMN, UMR Laboratoire de Chimie Biologie des Membranes et NanoObjets) ends with a publication  in  Plant Cell & Environment on the role of Diacylglycerol kinases in cryptogein signaling.
Thank you to everybody for these fruitfull collaborations and publications.


Cacas JL, Gerbeau-Pissot P, Fromentin J, Cantrel C, Thomas D, Emmanuelle J, Kalachova T, Mongrand S, Simon-Plas F & Ruelland E (2016) Plant Diacylglycerol kinases activate tobacco NADPH oxidase dependent oxidative burst in response to the PAMP cryptogein. Plant Cell & Environment, in press

[May 20, 2016] The LBM invited Eric Hosy (Institut Interdisciplinaire de Neurosciences, CNRS/Université de Bordeaux) through the invitation program of the FR BIE for a very interesting seminar entitled "How single particle based super-resolution techniques succeed to improve our vision of molecular dynamic organization of the cell". Thank you for the nice discussion on super-resolution microscopy.



[May 13, 2016] Juliette Jouhet from the Laboratoire de Physiologie Cellulaire et Végétale in Grenoble gave a seminar invited by Florence Corellou LBM and the FR BIE on "AtMic60 is involved in plant mitochondria lipid trafficking and is part of a large complex". Than you for the discussions on membrane contact sites.



[April 20, 2016]
Jerome and Fred, in collaboration with the team of Christophe Rothan from BFP unit in Bordeaux, participated in the characterization of a new tomato (Solanum lycopersicum) cutin mutant. The fruit cuticle thickness, composition and properties were altered in the gpat6 mutant. In vitro assays with recombinant proteins produced in yeast showed that a point mutation adjacent to the active site of the GPAT6 enzyme completely abolished its acyltransferase activity.

 

J. Petit, C. Bres, J.-P. Mauxion, F. Wong Jun Tai, L.B.B. Martin, E.A. Fich, J. Joubès, J.K.C. Rose, F. Domergue and C. Rothan (2016) The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis. Plant Physiol., doi:10.1104/pp.16.00409.


[April 6, 2016] Camille, Jérôme and Fred, in collaboration with Steven Moussu and Gwyneth Ingram from the RDP lab in Lyon, published a review,
in the book "Lipids in Plant and Algae Development" edited by Y. Nakamura and Y. Li-Beisson, on the relationship between plant surface lipids and epidermis development and the difficultly of functionally separate epidermal cell fate specification from cuticle biogenesis.  



Delude C.
, Moussu S., Joubès J., Ingram G. and Domergue F. (2016) Plant surface lipids and epidermis development. In "Lipids in Plant and Algae Development", "Subcellular Biochemistry" vol. 86, Nakamura Y. and Li-Beisson Y. (eds), Springer International Publishing Switzerland, pp 287-313.


[March 24, 2016] Yohann and Magali, in collaboration with the team of Shuzhen Men (College of life sciences, Nankai University, China), contributed to a publication in Plant Physiology on the functional role of an enzyme of the sterol biosynthesis pathway, the sterol 4α-methyl oxidase2 protein (SMO2)  in regulation of embryogenesis in Arabidopsis through auxin accumulation and PIN1 expression defect. This work strengthens the idea that precursors of bulk sterols are involved in plant development and that a correct balance between precursors and bulk sterols is critical for plants.


Xia Zhang, Shuangli Sun, Xiang Nie, Yohann Boutté, Magali Grison, Panpan Li, Susu Kuang, Shuzhen Men (2016). The Arabidopsis Sterol 4α-Methyl Oxidase2 Family is esssential for Auxin-Regulated Embryonic Development. Plant Physiology, in press.


[March 11, 2016] Invited by Sébastien Mongrand throught the FR BIE,  Sophie Layé (Nutrition and Integrative Neurobiology, INRA Bordeaux University) - Lauréate du Laurier Défi Scientifique 2015 - gave a very interesting seminar on "Nutritional Omega 3, what role in the brain?"



[March 10, 2016] Phytolongins are a new family of non-SNAREs longins in plant cells. Four proteins have been identified in Arabidopsis (Phyl 1.1, 1.2, 2.1 and 2.2) and their subcellular localization and trafficking has been determined in this work published by our team (Valerie Wattelet-Boyer, Guillaume Bouyssou, Claireline Marais, Yohann Boutté and Patrick Moreau ) in Journal of Experimental Botany with the strong collaboration of Carine de Marcos Lousa (University of Leeds-Becket) and Francesco Filippini (University of Padova).
In this study, we found that the four phytolongins are distributed along the secretory pathway, Phyl2.1 and Phyl2.2 were strictly located at the ER network, Phyl1.2 at the Golgi bodies and Phyl1.1 was mainly associated to the plasma membrane and partially to post-Golgi compartments. Our results show that Phyl1.1 export from the ER depends on the GTPase Sar1, the Sar1 guanine nucleotide exchange factor Sec12, and the SNAREs Sec22 and Memb11. In addition, we have identified the Y48F49 motif as being critical for the escape of Phyl1.1 from the ER. Our results provide the first characterization of the sub-cellular localization of this new class of longins called phytolongins and discuss their potential roles in membrane structure and trafficking.

Carine de Marcos Lousa, Eric Soubeyrand, Paolo Bolognese, Valerie Wattelet-Boyer, Guillaume Bouyssou, Claireline Marais, Yohann Boutté, Francesco Filippini and Patrick Moreau.
Subcellular localization and trafficking of phytolongins (non-SNARE longins) in the plant secretory pathway.
J. Exp. Bot. 2016, in press.

[January 11-12, 2016] Antoine Loquet and Erick Dufourc organised the Aquitaine-Liège Symposium on "Plant lipids, membranes and NMR" at IECB with LBM teams and biophysicists from Gembloux working on membranes: two very intereting days. Thank you for the organisation.



[January 4, 2016] Three new master 2 students enter the LBM: Anne-Flore Deroubaix with Véronique Germain, Adiilah Mamode Cassim with Sébastien Mongrand, and Amazigh Hamaï with Vincent Arondel. WELCOME ABOARD





Happy New Year

[December 22, 2015] François Doignon and his team demonstrated that phosphoinositides containing stearic acid are involved in the control of intracellular trafficking and polarity of the actin cytoskeleton. For the first time, a new picture of actin cytoskeleton with bipolar structures is revealed in psi1Δ strains deficient for phosphoinositides with stearic acid and in mutants affected for bipolar budding pattern.



Doignon F., Laquel P., Testet E., Tuphile K., Fouillen L. and Bessoule J.J. (2016) Requirement of phosphoinositides containing stearic acid to control cell polarity. Molecular and Cellular Biology, in press


[December 15, 2015] Camille Delude
defended her Ph. D. thesis directed by Frederic Domergue on the mechanism of suberin monomer export and the relevance of fatty alcohols in suberin biochemistry. The comitee was constituted by Bénédicte Bakan from INRA Nantes, Fred Beaudoin from Rothamsted Research, Gwyneth Ingram from ENS Lyon and Dominique Rolin from INRA Bordeaux. Congrats Dr Delude !


[December 11, 2015] Valérie Wattelet-Boyer
succesfully defends this afternoon her EPHE diploma (École Pratique des Hautes Études) on the role of sphingolipids in the secretory pathway during Arabidopsis root gravitropism. Congratulations !



[October 25-30, 2015] Manue Bayer attented the 11th international congress of plant molecular biology in Igassu / Brasil. She gave a seminar on plasmodesmata. Of course, she took the opportunity to visit the beautiful falls (with our colleague from Bordeaux, Yves Gibon).



[November 13, 2015] Manue Bayer thrue the FR BIE invited Waltraud SCHULTZE (Universität Hohenheim, Stuttgart) to talk of "Definition and functional analysis of membrane microdomains in plants". Nice talk. Thank you for coming !




[November 8, 2015]
Godswill Ntsomboh is the recipient of a short term EMBO fellowship and he arrived in the laboratory on October 19. He comes from the Center for research on oil palm (CEREPAH) of La Dibamba in Cameroon. He will stay in the laboratory for 3 months, to analyse palm oil. Welcome Godswill!



[November 2, 2015] QUIZZ: What is the main sphingolipid on earth? 

Glycosyl Inositol Phosphoryl Ceramides (GIPCs), of course !
Discover in our recent publication in Plant Physiology entitled "Re-visiting plant plasma membrane lipids in tobacco: a focus on sphingolipids", the putative role in plant membrane biology of these interesting lipids.



Cacas J.L., Buré C., Grosjean K., Gerbeau-Pissot P., Lherminier J., Rombouts Y., Maes E., Bossard C., Gronnier J., Furt F., Fouillen L., Germain V., Bayer E., Cluzet S., Robert F., Schmitter J.M., Deleu M., Lins L., Simon-Plas F., Mongrand S. (2015) Re-visiting plant plasma membrane lipids in tobacco: a focus on sphingolipids. Plant Physiology, in press

[October 5, 2015]
Fred Domergue attended last week the 13th Euro Fed Lipid Congress in Florence (Italy), for which he organized and chaired together with Ignace De Bruyne (ID&A, Belgium) the plant lipid sessions. Fred also gave two oral presentations on projects from his team.

[October 1st, 2015] Two new PhD students arrive at the LBM: Paul GOUGUET with Sébastien Mongrand to work on the plant raft protein REMORIN, and Nicolas ESNAY with Yohann Boutté to work on Trans Golgi Network and the secretory pathway. All the best for your thesis.


[September 1st, 2015] The LBM is happy to welcome several new collaborators, a new engineer, Françoise IMMEL from Dijon; a new technician, Karine TUPHILE from Orsay, and one new post-doc Amelia GASTON-DAUZON in the team of Manue Bayer to work on plasmodesmata.  WELCOME TO LBM !

[August 26, 2015] If you are interested by SNAREs and GTPases in the plant secretory pathway, you are invited to read our recent paper dealing with the specific interaction of the Qb-SNARE Memb11 with the GTPase Arf1 in the Golgi apparatus of Arabidopsis which is published in the Journal of Experimental Botany.



"The Qb-SNARE Memb11 interacts specifically with Arf1 in the Golgi apparatus of Arabidopsis thaliana" by Marais C, Wattelet-Boyer V, Bouyssou G, Hocquellet A, Dupuy JW, Batailler B, Brocard L, Boutté Y, Maneta-Peyret L and  Moreau P.


[July 5-9, 2015]
Meanwhile, Vincent and Claire attented the 26th ICAR (International Conference on Arabidopsis Research) in Paris.


[July 6-8, 2015] Camille, Charlotte, Florence, Fred and Sébastien came back from Rothamsted. They attended the 7th European Symposium on Plant Lipids in the newly opened conference centre on site at Rothamsted Research, the oldest agricultural research institute in the world, based in Harpenden, Hertfordshire, UK.
They visited during the meeting the  phenotypage facility of the research center and the first open-field of GM plants in UK (Camelina producing fish oil); top pictures.



[June 17, 2015] Houda Nacir defended her Ph. D. thesis directed by Claire Brehelin on the lipid composition of plastoglobules. The comitee was constituted by Hubert Schaller, Jean-Luc Montillet, Justine Bertrand-Michel, François, Laetitia and Claire. Congrats Dr Houda !



[June 16-20, 2015] Jérôme and Fred gave seminars at the Plant Waxes workshop in Switzerland where they had deep discussions with the paleoclimatologist/geochimist community on isotope proxies in ancient sedimentary archives.



[May 28, 2015] Manue invited Bruno Humbel (head of the Electron Microscopy Facility of the University of Lausanne) for a seminar on the state of the art Correlative Microscopy.



[May 28, 2015] Bruno Humbel, Jens Tislner (University of St Andrews, UK) and Sylvain Trepout (Institut Curie, Paris) came for the mid-term comitee of William Nicolas. Thank you very for the very interesting discussions.


[April 24, 2015] Cyril Zipfel came from The Sainsbury Laboratory to give a seminar on "Connecting the dots: linking PAMP perception to receptor kinase-mediated immune outputs" invited by the LBM through the FR BIE.



[April 25, 2015] Niko Geldner came from the University of Lausanne to talk about "The endodermis - a tale of two cell types "



Both Cyril and Niko came for the mid-term comitee of Julien Gronnier. Thank you very for the very interesting discussions.

[March 30, 2015]
What is the lipid composition of plasmodesma-membrane lined channels ? This question was finally tackled by several members of the LBM in a recent publication in The Plant Cell. In collaboration with colleagues in Leeds (UK) and Bonn (Germany) we showed for the first time that membrane lipids are important for PD function: Sterols modulate PD targeting of GPI-anchored proteins and change callose-mediated cell-to-cell connectivity . 
 


Grison M.S., Brocard L., Fouillen L., Nicolas W., Wewer V., Dörmann P., Nacir H., Alfonso-Benitez Y., Claverol S., Germain V., Boutté Y., Mongrand S. & Bayer E.M. (2015) Lateral segregation of lipids at primary plasmodesmata defines plasmodesmal localisation of two GPI-anchored proteins in arabidopsis. The Plant Cell, in press



[March 30, 2015] Jean-Jacques in collaboration with Tom Roscoe and Martine Devic (IRD, UMR DIADE, Montpellier), contributed to a publication in Plant Cell Physiology on ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2 (LEC2), collectively the AFL, that are master regulators of seed maturation processes.
The  study examined the role of AFL in the production of seed reserves in Arabidopsis. Quantification  of seed reserves and cytological observations of afl  mutant embryos show that protein and lipid but not starch reserves are spatially regulated by AFL. Although AFL contribute to a common regulation of reserves, ABI3 exerts a quantitatively greater control over storage protein content whereas FUS3 controls lipid content to a greater extent. Whereas ABI3 controls the reserve content throughout the embryo, LEC2 and FUS3 regulate reserves in distinct embryonic territories
 
 
Roscoe et al (2015) Complementation of Seed Maturation Phenotypes by Ectopic Expression of ABSCISSIC ACID INSENSITIVE3, FUSCA3 and
LEAFY COTYLEDON2 in Arabidopsis. Plant Cell Physiol, in press


[February 25, 2015] Lilly and Patrick collaborated with the team of Alessandra Moscatelli (University of Milano) to characterize isolated membrane domains from pollen tubes of Nicotiana tabacum.



Moscatelli A, Gagliardi A, Maneta-Peyret L, Bini L, Stroppa N, Onelli E, Landi C, Scali M, Idilli AI, Moreau P. (2015) Characterisation of detergent-insoluble membranes in pollen tubes of Nicotiana tabacum (L.). Biol Open. 2015, doi: 10.1242/bio.201410249.

[February 2-6, 2015] Manue gave a seminar at the Gordon Conference on Plant Lipids, in Texas : Linking Plasmodesmata Membrane Lipid Composition and Cell-to-Cell Connectivity in Plants...and took the opportunity to visit the NASA's Space Center in Houston !
After working on plasmodesma, she plans to be an astronaut !



[January 19, 2015] Jérôme and the wax team members, in collaboration with the Team of Ljerka Kunst (UBC Vancouver), contributed to a publication in Plant Physiology on the functional characterization of the CER2-LIKE proteins and their importance for cuticle formation and pollen coat function.
Haslam T.M., Haslam R., Thoraval D., Pascal S., Delude C., Domergue F., Manas-Fernandez A., Beaudoin F., Napier J.A., Kunst L. and Joubès J. (2015). CER2-LIKE proteins have unique biochemical roles and physiological functions in very-long-chain fatty acid elongation.

cer2 cer2-like1 cer2-like2 pollen grain

[January 15, 2015] Mathieu, a research engineer, is going to work on the comparative proteomic analysis of plasmodesmata fraction together with Emmanuelle and William. Welcome in the LBM.



[January 14, 2015] The LBM welcomes our new Master 2 students, Paul, Nicolas, Vanessa & Paul who are going to work with Véronique, Claire, Yohann and Jérôme, respectively.




[January 7, 2015] ......................




[December 19, 2014] Guillaume, Laetitia and Patrick contributed to a publication in "PLoS One" with Michael Boshart's team (University of Munich) and Frédéric Bringaud (University of Bordeaux) on the storage of TAG in the lipid droplets of the procyclic forms of Trypanosoma brucei.
Allmann S, Mazet M, Ziebart N, Bouyssou G, Fouillen L, Dupuy JW, Bonneu M, Moreau P, Bringaud F and Boshart M (2014) Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei. PLoS ONE 9(12): e114628.

Our report shows that feeding of T. brucei with oleate resulted in a 4–5 fold increase in the number of lipid droplets, as quantified by confocal fluorescence microscopy and by flow cytometry of BODIPY 493/503-stained cells. The TAG content also increased 4–5 fold, and labeled oleate was incorporated into TAG. A single candidate gene, TFEa1  coding for a subunit of the trifunctional enzyme complex was identified. Unexpectedly, a TFEa1 gene knock-out mutant still expressed wild-type levels of previously reported NADP-dependent 3-hydroxyacyl-CoA dehydrogenase activity, and therefore, another gene must encode this enzymatic activity. The absence of net catabolism of stored TAG in procyclic T. brucei did not formally exclude a flux through TAG, in which biosynthesis equals catabolism. Also, the possibility remains that TAG catabolism is completely repressed by other carbon sources in culture media or developmentally activated in post-procyclic stages in the tsetse fly.



[December 13, 2015] Our long-lasting collaboration with the team of Françoise Simon-Plas and Patricia Gerbeau-Pissot, in the university of Burgundy-INRA is still very vigorous !
The effect of the wide variety of plant lipids on membrane organization is still poorly understood. We showed in that paper that the local amount of phytosphingolipids (GIPC and gluCER), and free- and conjugated-phytosterols control the relative proportion and spatial distribution of ordered domains, and plant lipids differently modulate, alone or in combination, membrane heterogeneity. Our results highlight how plant lipid diversity might drive the plasma membrane sub-compartmentalization.

Kevin Grosjean, Sébastien Mongrand, Laurent Beney, Françoise Simon-Plas & Patricia Gerbeau-Pissot (2015) Differential effect of plant lipids on membrane organization: hot features and specificities of phytosphingolipids and phytosterols. The Journal of Biological Chemistry, in press

[December 8, 2014] In collaboration with the Team of T. Noel (UMR 5234) and and S. Manon (UMR 5095), JJ Bessoule contributed to a publication in "PLoS One" fatty acid ß-oxidation pathway in the yeast Candida lusitaniae
F.  Gabriel, I Accoceberry, JJ Bessoule, B. Salin M. Lucas-Guerin, S. Manon, K. Dementhon, T. Noel (2014) A Fox2-Dependent Fatty Acid ß-Oxidation Pathway Coexists Both in Peroxisomes andMitochondria of the Ascomycete Yeast Candida lusitaniae.PLOS ONE | DOI:10.1371/journal.pone.0114531, December 8, 2014
Whereas it was generally admitted that the ascomycete yeasts of the subphylum Saccharomycotina possess a single fatty acid ß-oxidation pathway located exclusively in peroxisomes, and that they lost mitochondrial ß-oxidation early during evolution, we showed that in the opportunistic pathogenic yeast Candida lusitaniae, fatty acid catabolism takes place in both peroxisomal and mitochondrial subcellular fractions.

[December 2-5, 2014] LEB Aquitaine Transfert participated as an exhibitor at
Pollutec in Lyon. This exhibition helped LEB Aquitaine Transfert to be better known. The Omega-3 Index to diagnose soil pollution developed by LEB Aquitaine Transfert was selected among the best innovations of the exhibition by Environment Magazine. This magazine wrote an article about this tool published in the issue 1732 of November (http://archives.environnement-magazine.fr/content/system/media/1/1732/page_0091.pdf).



[November 18-19, 2014] Marina and Jean-Jacques participated at the 3rd meeting of the Research on Polluted Sites and Soils organized by ADEME in Paris. Marina gave a talk entitled “Omega-3 Index: from theory to the applicability of this soil pollution diagnostic tool.”



[November 19-21, 2014] Laetitia attended to the Merlion Metabolomics Workshop 2014 (Singapore), with the MetaboHub delegation. She gave a talk entitled "triacyglycerols Lipidomics: Application to palm oil".



[November 15-19, 2014] Very inspiring conference Jacques Monod held in Roscoff Brittany France on the organization and dynamics of biological membranes. Emmanuelle Bayer form the LBM was selected for a oral presentation on "the role of lipids in the regulation of cell to cell communication in plants through the nano-scaled channel plasmodesmata". She met Emmanuel Lemichez (Nice) and Eric Maréchal (Grenoble) here on the picture, to enjoy Science and Sea food !



[November 21, 2014] Fred and Vincent participated at the Symposium on Synthetic Biology organized by SB2 in Bordeaux.


[October, 30 2014] The LBM wishes an happy retirement to Annie Claveres. Congratulations, may you enjoy your retirement to the fullest. We'll miss you !



[October 6, 2014] The LBM is glad to welcome its new PhD student Charlotte DEGRAEVE-GUILBAULT supervised by Florence. The title of her thesis is "Biosynthesis & role of DHA for membrane physiology in the model microalgae Ostreococcus tauri". Good luck for your work!



[September 24, 2014]
Fred and Vincent attended to the 12th EuroFed Lipid meeting in Montpellier (sept 14-17th). This meeting, which dealt with all aspect of lipids from plant breeding to human nutrition and industrial processing of fats, gathered more than 900 participants and had a nice plant lipid session. Vincent gave a lecture on oil palm lipase and Fred presented a poster on Wax Esters.



[September 24, 2014] Denis participated to a study entitled "Incorporation of therapeutically modified bacteria into gut microbiota inhibits obesity". Indeed N-acylphosphatidylethanolamines (NAPEs) are precursors to the N-acylethanolamide (NAE) family of lipids, which are synthesized in the small intestine in response to feeding and reduce food intake and obesity. Here, we demonstrated that administration of engineered NAPE-expressing E. coli Nissle 1917 bacteria in drinking water for 8 weeks reduced the levels of obesity in mice fed a high-fat diet. Our results demonstrate that incorporation of appropriately modified bacteria into the gut microbiota has potential as an effective strategy to inhibit the development of metabolic disorders.
Chen, Z., Guo, L., Zhang, Y., L. Walzem R, Pendergast, J. S., Printz, R. L., Morris, L. C., Matafonova, E., Stien, X., Kang, L., Coulon, D., McGuinness, O. P., Niswender, K. D., Davies, S. S. (2014) Incorporation of therapeutically modified bacteria into gut microbiota inhibits obesity. J Clin Invest, 124, 3391-406.

[September 23, 2014] Sébastien and Jean-Luc Cacas (a former post-doc of the LBM) participated to a study entitled "Direct purification of detergent-insoluble membranes from Medicago truncatula root microsomes" recently accepted in BMC plant biology. A very usefull protocol for raft purification.
Guillier C, Cacas JL, Recorbet G, Deprêtre N, Mounier A, Mongrand S, Simon-Plas F, Wipf D & Dumas-Gaudot E (2014)  Direct purification of detergent-insoluble membranes from Medicago truncatula root microsomes: Comparison between floatation and sedimentation. BMC Plant Biol., in press



[September 19, 2014]
If you are interested by the roles that lipids can play in the function of the plant secretory pathway, you are invited to read our recent review “Modulation of endomembranes morphodynamics in the secretory/retrograde pathways depends on lipid diversity” which is published in Current Opinion in Plant Biology (2014, vol 22: 22-29).




[September 15, 2014] Yohann and Patrick came back from the annual meeting of the European Network for Plant Endomembrane Research in Lecce, Italy. As usual this meeting provided updated advances in the field of endomembrane trafficking. Yohann did a talk on the role of lipids in sorting of auxin carriers at post-Golgi using gravitropism as a readout.


[September 15, 2014] If you want to known more about post-Golgi functioning, associated molecular mecanisms and post-Golgi function in plant development, we could only invite you to read our recent review entitled "Journey to the cell surface-the central role of the trans-Golgi network in plants" published in Protoplasma.



[September 15, 2014] Together with Thomas Stanislas and Markus Grebe, Yohann also published a method paper on visualization of sterols during endocytic trafficking



[September 12, 2014] The LBM says goodbye to Glawdys MARIE-LUCE after her rotation with Florence to work on Ostreococcus lipids. Thank you for the nice drink, and good luck for everything !



[July 6-11, 2014] Fred attended the 21st International Symposium on Plant Lipids 2014 at the University of Guelph (which has a gryphon as mascot) in Ontario, Canada. Once again, the plant surface lipid sessions were most interesting!!!



[August 24-29, 2014] Manue, Véro and William came back from the very interesting EMBO workshop meeting: "Intercellular communication in plant development and disease" in Bischoffsheim (near Strasbourg), France. More than hundred people attented. Manue gave a talk entitled "Lateral segregation of plasma membrane lipids across plasmodesmata cell boundary: a role for lipids in the regulation of intercellular trafficking?".




[July 23, 2014] With E. Blee’s and I. Feussner’s Labs, Marina and Jean-Jacques published a paper about RD20, a caleosin with an unusual peroxygenase activity. RD20 overexpressing Arabidopsis plants exhibit elevated levels of reactive oxygen species associated with early gibberellin-dependent flowering and abscisic acid hypersensitivity. RD20 confers also tolerance against stress induced by paraquat, Rose Bengal, heavy metal and synthetic auxins.

Blée E, Boachon B, Burcklen M, Le Guédard M, Hanano A, Ehlting J, Herrfurth C, Feussner I, and Bessoule JJ. (2014) The reductase activity of Arabidopsis caleosin RD20 mediates GA-dependent flowering time, abscisic acid sensitivity and tolerance to oxidative stress. Plant Physiol, in Press

[July 14, 2014] With F. Brandizzi's Lab (Michigan State University, Michigan, USA), Lilly, Laetitia and Patrick published a paper on RHD3, a member of the ER-shaping dynamin-like GTPases. They show that a deregulation of phospholipid synthesis occurs in rhd3 mutants and that it can be compensated in a pis1 (PI synthase 1) mutant, suggesting that RHD3 could have some link with PIs1 and lipid metabolism.
Lilly Maneta-Peyret, Ya-Shiuan Lai, Giovanni Stefano, Laetitia Fouillen, Federica Brandizzi and Patrick Moreau (2014). Phospholipid biosynthesis increases in RHD3-defective mutants. Plant Signaling Behavior, in press.

Seb came back from the SEB meeting [Society for Experimental Biology] in Manchester. He did the opening lecture on "How do plant proteins interact with membranes?" and even found the perfect place to work on REMORIN: The REM bar in Manchester !



[June 27, 2014] LBM invited two researchers from Rothamsted Research Center in UK: Dr. Olga Sayanova "Designing algal oils: a new platform for the sustainable production of high-value lipids" and Dr. Fred Beaudoin «Manipulating epicuticular wax structure and composition to improve photosynthesis and increase the yield of Brassica oilseed crops».
Thank you very much for coming.



[June 17, 2014]
The LBM Surface Lipid team does not only work on plant lipids !!! In this study, we characterized TtFARAT, a bifunctional protein from Tetrahymena thermophyla which catalyzes the formation of the two precursors required to initiate ether lipid biosynthesis in the peroxisomes. The N-terminal part is a Fatty Acyl-CoA Reductase (FAR) while the C-terminal domain is an Acyl-Transferase (AT).

Dittrich-Domergue F., Joubès J., Moreau P., Lessire R., Stymne S. and Domergue F. (2014). The bifunctional protein TtFARAT from Tetrahymena thermophyla catalyzes the formation of both precursors required to initiate ether lipids biosynthesis. The Journal of Biological Chemistry, in press.

[April 24, 2014] REMORIN STRIKES BACK. Sylvain Raffaele, a former post-doc of the LBM, demonstrated the role of REM in the suceptibility to Phytophthora infestans, the oomycete that causes the serious potato disease known as late blight or potato blight. By contast with virus, REM enhances susceptibility to Phytophthora infestans. This work is published in Plant Physiology, and Sébastien collaborated in this study.

Bozkurt TO., Richardson A., Dagdas YF, Mongrand S., Kamoun S., and Raffaele S. (2014) The plant membrane protein REM1.3 remorin accumulates in discrete extrahaustorial membrane domains and enhances susceptibility to Phytophthora infestans. Plant physiology, in press.

We did the cover of
Plant Physiology for this paper !!



[April 9-10, 2014] Laetitia from the LBM organized  with the LipidomYst french network, two days in Bordeaux at the CGFB to discuss Surface lipids and Methods for lipid extraction. 40 people was attented to this meeting sponsored by ABsciex.



[April 7, 2014] The 11th edition of the French conference "Yeast Models and Tools" was held in Bordeaux from 7 to 9 April 2014, many members of LBM were present.



[March 6, 2014] The raft team of the LBM together with their colleagues from Buenos Aires and Bordeaux, investigated deeper the role of REMORIN, a plant plasma membrane protein located in microdomain enriched in sterols. The antiviral effect of Remorin and its potential role as a general regulator of intercellular communication in plants is proposed in this work published in FEBS Letters.



StRemorin1.3 hampers Potato virus X TGBp1 ability to increase Plasmodesmata permeability, but does not interfere with its silencing suppressor activity (2014) Artemis Perraki, Maria Binaghi, Martin M Mecchia, Julien Gronnier, Sylvie German-Retana, Sébastien Mongrand, Emmanuelle Bayer, Alicia M Zelada, Véronique Germain. FEBS Letters

[February, 4 2014] Manue and Seb  from the raft team together with Jens Tilsner, University of St Andrews and Lesley Torrance, The James Hutton Institute in UK were Topics editors of a special issue about plasmodesmata in Frontiers in Plant Science named: "Specialised membrane domains of plasmodesmata, plant intercellular nanopores". Have a look by clicking here:
 http://www.frontiersin.org/Plant_Cell_Biology/researchtopics/Specialised_membrane_domains_o/1470



[January 30, 2014] Asma Fourati, a student from Sousse Polytechnique school in Tunisia and Celine Diaz, from CRAG in Barcelona, will work a few months in the lab with Vincent Arondel

[January 29, 2014] Francois from the LBM participates to a study on Helicobacter pullorum. This bacterium, initially isolated from poultry, has been associated with human digestive disorders. However, the factor responsible for its cytopathogenic effects on epithelial cells has not been formally identified. All of the H. pullorum strains induced cellular distending phenotype, actin cytoskeleton remodeling. The cytolethal distending toxin (CDT) family of H. pullorum toxins  is thus responsible for major cytopathogenic effects in vitro, confirming its role as a main virulence factor of this emerging human pathogen.
Varon C. et al. (2014). Helicobacter pullorum Cytolethal Distending Toxin Targets Vinculin and Cortactin and Triggers Formation of Lamellipodia in Intestinal Epithelial Cells. J. Infect. Dis., 209, 588.



[January 14, 2014] To celebrate 2014, LBM members organize in the lab an international cooking contest. Delicious and funny !



[September 1st, 2013] The LBM welcomes a new CNRS colleague: Patricia Laquel, AKA Pathy. Pathy will work on yeast membrane polarity in Francois Doignon's team.



[January 16, 2014]
The LBM welcomes two students to work with Claire and Laetitia: Lucie Combes-Soia in Master and Lionelle Belonga in BTS.

 

The LBM wishes you a Happy New Year 2014

[December 20, 2013] Frédéric
and Jérôme from the Wax Team contributed to a publication in Plant Physiology with Christophe Rothan and his team of the UMR 1332 Fruit Biology & Pathology at INRA Bordeaux. In this work, they screened an EMS mutant collection in the miniature tomato cultivar Micro-Tom for mutants with altered fruit brightness to further decipher the relationships between fruit brightness and cuticle in tomato fruit.
Petit et al., Plant Physiology


[December 16, 2013] Claireline Marais defends her thesis work on the role of membrin in plants. The comitee was comprised of Nathalie Leborgne-Castel, Maryline Vantard, Nadine Paris, François Doignon and her thesis director Patrick Moreau. Good luck for the future !



[December, 10 2013] Jérôme Joubès from the Wax team 
defended his "Habilitation à Diriger les Recherches" on Functional Genomics of Wax Biosynthesis in Plants with JD. Faure, F. Pinot, Y. Li-Beisson, E. Gomes and C. Chevalier in his comitee. Congratulations Jérôme !



[December, 10 2013] In the morning, Dr Yonghua Li-Beisson from CEA Cadarach gave a seminar on “Screens, mutants, and –omics approaches toward understanding of lipid metabolic pathways in the algae Chlamydomonas reinhardtii”.



[December 9, 2013] It is the turn of Magali Grison to succesfully pass her EPHE diploma on the involvement of lipids in the plasmodesmal structure.
  
YOU ARE THE BEST, GIRLS!



[December 6, 12013]
Stéphanie Pascal succesfully defends this afternoon her EPHE diploma (École Pratique des Hautes Études) on the functional characterization of the CER2 gene family and their role in cuticular wax biosynthesis. Congratulations !



[November 29, 2013]. Our neigbhour Jean-Paul Douliez from the UMR Biologie du Fruit & Pathologie came to the LBM to explain us how to make micelles with free fatty acids !! Very intersting talk. Thank you for coming.



[November 18, 12013] The group of our long-time collaborators from Dijon published in Plant Physiology with Sébastien from the LBM  that the plasma membrane of tobacco suspension cells contains small areas exhibiting a wide range of physical states. Its organization, both in term of fluidity and order level, is modified during the early steps of the signaling cascade induced by an elicitor of defense reactions. Check that results emphasizing the role of membrane domains in signalling pathways in plants.



[November 7, 2013] Guillaume and Patrick from the LBM contributed to a publication in "PLOS Neglected Tropical Diseases" with Frédéric Bringaud and his team (CRMSB, UMR-5536 CNRS-Université Bordeaux Segalen).
It was generally accepted that pyruvate is the major end-product excreted from glucose metabolism by the bloodstream forms of Trypanosoma brucei with virtually no production of succinate and acetate, the main end-products excreted from glycolysis by the procyclic insect form of T. brucei. The work shows that mitochondrial production of acetate is essential for the bloodstream forms, since blocking of acetate biosynthesis from both carbon sources induces cell death. In addition, we have shown that de novo fatty acid biosynthesis from acetate is essential for this parasite, as demonstrated by a lethal phenotype and metabolic analyses of RNAi-mediated depletion of acetyl-CoA synthetase. Consequently, this metabolic pathway could constitute new attractive drug targets against trypanosomiasis.
Mazet et al., PLOS Neglected Tropical Diseases, in press.

[November 4, 2013]



[November 4, 2013]
Lilly and Patrick from the LBM participated to a publication in Plant Cell governed by Antoine Danon from the "Institut de Biologie des Plantes, Université de Paris-Sud XI, CNRS, UMR 8618, Orsay". This study investigated the importance of the Cardiolipin Synthase in several aspetcs of plant mitochondria: mitochondrial ultrastructure, respiratory function, plant development, and stress responses with Arabidopsis as the plant model. Pineau et al., Plant Cell, in press.

[September 19, 2013 ] Franco Puccio stayed in the lab for one month. He comes from Buenos Aires in the exchange program ECOS-SUD France-Argentina  with Alicia Zelada's lab. On the picture : Seb, Véro, Sylvie from the plant virology lab INRA Bordeaux, and Franco.



[September 18, 2013]
Yohann Boutté published a first-author paper in PNAS revealing distinct mechanisms for secretory trafficking of auxin carriers from the post-Golgi compartment trans-Golgi network to the plasma membrane. Yohann also published a co-author paper in Plant Cell on Golgi-independent trafficking of proteins and lipids from the endoplasmic reticulum to the vacuole.



[September 16, 2013] Laetitia and Sebastien came back from the kick-off meeting of the project FIELD in Gembloux, Belgium. This opens a very promising collaboration with chemists, biophysists, phytopathologists and plant biologists.


[September 12, 2013] Marina and Jean-Jacques attended to the 7th conference of the European Plant Science Organisation, 1-4 september 2013, Porto Heli (Greece)



[September 9, 2013] The collaboration between Owen Rowland (Carleton University, Ottawa, Canada) and Fred Domergue produced two new papers on Fatty Acyl Reductases (FARs)!!!
- Chacon M.G., Fournier A.E., Tran F., Dittrich-Domergue F., Pulsifer I.P., Domergue F. and Rowland O. (2013) Identification of Amino Acids Conferring Chain-Length Substrate Specificities on Fatty Alcohol-Forming Reductases FAR5 and FAR8 from Arabidopsis thaliana published in J Biol Chem. Using yeast as heterologous system, domain swapping and site-directed mutagenesis, this work identified by in vivo and in vitro approaches amino acids 355 and 377 as determinants for 16:0 versus 18:0 chain-length specificity in FAR5 and FAR8.
- Vishwanath S.J., Kosma D.K., Pulsifer I.P., Scandola S., Pascal S., Joubès J., Dittrich-Domergue F., Lessire R., Rowland O. and Domergue F. (2013) Suberin-Associated Fatty Alcohols in Arabidopsis thaliana: Distributions in Roots and Contributions to Seed Coat Barrier Properties published in Plant Physiology.  This study shows that most of the root fatty alcohols are probably non-covalently associated with the suberin polymer, and that reduced levels of fatty alcohols in seed coat suberin increase permeability to tetrazolium salts and sensitivity to abscisic acid.
What a fruitful collaboration!!

[September 9, 2013] Jerome and Fred
came back from Lausanne in Switzerland where they participated to the first International Symposium on Plant Apoplastic Diffusion Barriers: Biosynthesis and Functions (PADiBa 2013) organized by Dr Christiane Nawrath and Prof. Niko Geldner.


[September 1, 2013] The LBM welcomes a new colleague, Florence Corellou (CR1-CNRS), who will develop a project on Ostreococcus tauri membrane signaling by histine kinases in Sebastien's team.



[August 27, 2013] Eric and Laetitia are co-authors of a paper published in BBA-Molecular Basis of Disease, a work lead by Pascale de Lonlay (Inserm U781) in Paris. They demonstrate a considerable free fatty acid accumulation in the human lipin-1 deficient myoblast.



[August 23, 2013]
Guillaume and Patrick from the LBM contributed to a publication in Molecular Microbiology with Frédéric Bringaud and his team (Centre de Résonance Magnétique des Systèmes Biologiques, UMR-5536 CNRS-Université Bordeaux Segalen). This study shows that the procyclic trypanosomes grown in rich medium only use glucose and threonine for lipid biosynthesis, with threonine’s contribution being ~2.5-times higher than that of glucose. Acetate, which feeds lipid biosynthesis, is also produced primarily from threonine. Interestingly, the first enzymatic step of the threonine degradation pathway, threonine dehydrogenase (TDH, EC 1.1.1.103), is under metabolic control and plays a key role in the rate of catabolism. TDH expression is under control and can be down-regulated in response to metabolic perturbations, such as in the PEPCK mutant in which the glycolytic metabolic flux was redirected towards acetate production. The threonine degradation pathway of the Trypanosoma brucei procyclic form: the main carbon source for lipid biosynthesis is under metabolic control.
Yohann Millerioux, Charles Ebikeme, Marc Biran, Pauline Morand, Guillaume Bouyssou, Isabel M. Vincent, Muriel Mazet, Loïc Riviere, Jean-Michel Franconi, Richard J. S. Burchmore, Patrick Moreau, Michael P. Barrett and Frédéric Bringaud. Molecular Microbiology, in press.




[August 23, 2013]
Sebastien's team and collaborators analysed the polar head composition of glycosyl inositolphosphoceramides (GIPC) extracted from 23 plant species ranging from algae to monocots and dicots selected in different taxa. This biochemical survey, published in Phytochemistry, unravels an unexpected broad diversity and the occurrence of yet-unreported GIPC structures in green and red algae.
Jean-Luc Cacas, Corinne Buré, Fabienne Furt, Jean-Paul Maalouf, Alain Badoc, Stéphanie Cluzet, Jean-Marie Schmitter, Elvire Antajan, Sébastien Mongrand (2013) Biochemical survey of the polar head of plant glycosylinositolphosphoceramides unravels broad diversity. Phytochemistry





[July 7-10, 2013] The LBM organized the Eurofed Lipid symposium. 150 participants participated to this great event.


[July 16, 2013] The results of a collaborative project led by V. Arondel that aimed at improving palm oil quality are being published today in Nature Communications. Vincent and his colleagues from IRD, CIRAD, PalmElit, IRAD (Cameroon) and INRAB (Benin) identified the mesocarp lipase known since 1910 to deteriorate palm oil. They showed that low lipase lines of palm produce oil with less free fatty acids, allowing more flexibility for post-harvest fruit processing and extended ripening for increased yields. Expected benefits concern all planters but are likely to be highest for African smallholders who would be more able to produce oil that meets international quality standards.
Improving palm oil quality through identification and mapping of the lipase gene causing oil deterioration, Fabienne Morcillo, David Cros, Norbert Billotte, Georges-Frank Ngando-Ebongue, Hubert Domonhédo, Maxime Pizot, Teresa Cuéllar, Sandra Espéout, Rabeb Dhouib, Fabienne Bourgis, Stéphane Claverol, Timothy Tranbarger, Bruno Nouy, Vincent Arondel, Nature Communications (2013), doi:10.1038/ncomms3160.



[July 1st, 2013] The role played by the cuticle in drought stress adaptation was investigated in maritime pine using a factorial design with two genetic units and two treatments (irrigated vs non-irrigated), in field conditions. A higher cuticular wax content was observed in the non-irrigated treatment and associated with the over expression of several genes involved in cuticle biogenesis. This study suggests that cuticular waxes may be involved in drought stress adaptation in maritime pine as well as provides a set of promising candidate genes for future forward genetic studies in conifers.
Le Provost G., Domergue F. et al. (2013) Soil water stress affects both cuticular wax content and cuticle-related gene expression in young saplings of maritime pine (Pinus pinaster Ait). BMC Plant Biology (in press).



[July 1st, 2013] The ANR project "INASEED", coordinated by our colleague Gwyneth Ingram from the ENS-Lyon and having the "Surface Lipids Team" of the LBM as second partner, has been accepted. This project will study a novel signaling pathway involving an endosperm specific protease (ALE1), and two embryo specific receptor kinases (GSO1 and GSO2), which are necessary for the formation of a functional embryonic cuticle, and for the stabilization of embryonic epidermal cell fate. A multidisciplinary approach will be used to not only dissect in detail an entirely new and very striking example of how immune responses may have been harnessed by plants in order to fulfill developmental roles during the evolution of increasingly complex organs, but also provide novel and much needed understanding of both the composition of the embryonic cuticle, and of the effectors necessary for its biogenesis.



[June 13, 2013] Congratulations to our M2 students who defended their diploma today : Elise Thalineau worked on palm oil synthesis with Vincent ArondelJulien Gronnier worked on Remorin with Véronique Germain and William Nicolas worked with Emmanuelle Bayer on plasmodesmata (MASTER).



[May 31, 2013] SFR program invited through Manue Bayer of the LBM, Jens Tilsner from School of Biology, University of St Andrews UK. His talk was entilted "Spread globally, replicate locally – a new model for virus movement in plants". Very interesting discussions follow.



[May 30, 2013] Doan-Trung LUU from the  Biochimie & Physiologie Moleculaire des Plantes' lab in Montpellier gave a very nice seminar this morning on Intracellular trafficking of aquaporins in Arabidopsis thaliana. Thank you for coming in Bordeaux. Very interesting indeed.



[May 31, 2013]
Dictyostelium discoideum amoebae produce and secrete multilamellar bodies (MLBs), which are composed of membranous material when they are fed with bacteria. The role of the bacteria and the amoeba in the synthesis of MBLs constituents is not elucidated. In a research collaborative program with S. Charette’lab (University of Laval, Canada), René, Fred and Laetitia have demonstrated that the phospholipid and fatty acid compositions of MBLs are similar to amoeba and differ to bacteria. These results strongly suggest that the lipids constituting the MLBs originate from the amoebal metabolism and were not incorporated as a whole from the bacterial membranes (Paquet et al., Lipid composition of multilamellar bodies secreted by Dictyostelium discoideum reveals their amoebal origin, Eukaryotic Cell, 2013).



[May 29, 2013]
In collaboration with Ecolab (UMR 5245 CNRS, Toulouse), Marina and Jean-Jacques showed that the leaf fatty acid composition from L. sativa species makes it possible to determine qualitative soil-plant and/or air-plant field exposures to a complex mix of process particles enriched with metals and metalloids. The independence between the two absorption pathways with an additive effect in terms of phytotoxicity was demonstrated (Schreck et al, 2013, Environmental Pollution, 179, 242-249).

[April 26, 2013]  Frédéric Domergue
from wax team defended his "Habilitation à Diriger les Recherches" and gave a seminar unititled  "Diverses recherches sur des lipides des plantes : des Omega-3 aux lipides de surface".
Congratulations Fred.

[April 3, 2013] The  International Workshop on Plant Membrane Biology (IWPMB2013) held in Kurashiki, a beautiful city in Japan. Sébastien gave a seminar on the membrane raft phosphoprotein REMORIN. He was joined by his colleages from Dijon university: Francoise Simon and Daniel Wipf.




[February 13, 2013]
Microbial, plant and animal species were examined within the framework of the ADEME Bioindicator Program that involves the monitoring, characterization and risk assessment of soils from various sites. Whenever any of the selected species under study were not found in all of the sampling areas of a given site, the data were difficult to analyse for several groups involved in the Bioindicator Program. Marina and Jean-Jacques published a method to prevent discrepancies in interpretation in which a bio-value ("soil quality") is attributed to various areas, allowing them to be ranked. The method also provides an evaluation of the soundness of the results and the resulting ranking. (Le Guédard M. & Bessoule. J.-J., Ecological Indicators, In press).



[February 7, 2013] Jérôme
and Stéphanie from the LBM published with their collaborators, in the Plant Journal, the characterization of a new Arabidopsis eceriferum mutant named cer26, they notably showed that CER26 facilitates the elongation of the very-long-chain fatty acids with 30 carbons or more, with high tissular and substrate specificity (The Arabidopsis cer26 mutant, like the cer2 mutant, is specifically affected in the very-long-chain fatty acid elongation process).

[January 24, 2013] The LBM welcome several M2 students : Elise Thalineau who is working on palm oil synthesis with Vincent ArondelJulien Gronnier who is working on Remorin with Véronique Germain and William Nicolas who is working with Emmanuelle Bayer on plasmodesmata.
The LBM wishes you a Happy New Year 2013

[December 17, 2012]  Artémis Perraki from the Raft group get today her phD diploma ! congratulations.



[December 17, 2012] Jérôme
from the LBM published with Sebastien Baud and his team of the Institut Jean-Pierre Bourgin in INRA Versailles a Plant Cell paper. This study shows that the WRINKLED1 closed homologue genes WRI3 and WRI4 are required in floral tissues to provide acyl chains for cutin biosynthesis and prevent adherence of these developing organs and subsequent semisterility. The targets of these WRIs encode enzymes providing precursors (acyl chain and glycerol backbones) for various lipid biosynthetic pathways, but not the subsequent lipid-assembling enzymes. These results provide insights into the developmental regulation of fatty acidproduction in plants (link).

[December 3rd, 2012] Seven people from the LBM (Patrick, Yohann, Magalie, Véronique, Sébastien, Artemis and Emmanuelle) came back from Dijon where they attended the great meeting "Dijon Domains 2012" in presence of major scientists of the field like Kai Simons, Erwin London, Enrico Gratton, Katharina Gauss and so many others... We all learned a lot on the most advanced methodologies allowing the study of membrane sub-compartmentation, the latest exciting discoveries concerning the characterization of membrane organization, and the recent evidences indicating a crucial role of this organization and its dynamics in signaling processes and cell physiology in animals, plants and yeasts.



[November 22th, 2012]  If you want to know everything about Wax biosynthesis in Arabidopsis, you can read the review written by Amélie and Jérôme in Progress in Lipid Research.



[November 8th, 2012]. In the online CNRS journal,
Novembre-Décembre 2012. The article "Les levures : futurs gisements d'hydrocarbure" (p.9) comes back on the work of the LBM wax team led by René Lessire.



[November 8th, 2012] Jean-Paul Maalouf from the ANASTATS came to the lab to present his company specialized in statistical analyses. Thank you for this nice seminar.


[Novembre 5th, 2012] Laetitia  published her post-doc paper in Journal of Proteomics about Neuropeptide alterations in the (cute!) tree shrew hypothalamus during volatile anesthesia. That is the final paper of her postdoc story in the Pr. Rainer's lab (Visual Cognition Laboratory, university of Fribourg, Switzerland).


[August 29-31, 2012] We came back from the 15th ENPER (European Network for Plant Endomembrane Research) meeting in Madrid, organized by our colleagues Enrique Rojo, Michael Sauer and Jan Zouhar! Three people from the LBM (Claireline Marais, Yohann Boutté and Patrick Moreau ) attended this International Symposium. Claireline presented her current PhD work on "The ER-Golgi SNARE Memb11: also a receptor for the COPI-GTPase ARF1?" and Yohann Boutté exposed his postdoctoral work in R. Bhalerao's lab made in Umea on the protein ECHIDNA.

[October 29th, 2012] Invited by Frédéric Domergue, Dr Gwyneth C. INGRAM from the ENS Lyon - UMR 5667 
«Reproduction et développement des plantes» will give a seminar entitled "Embryonic cuticle formation impacts epidermal specification pathways in Arabidopsis thaliana" at the IBVM the 13th of November.

[October 21-26, 2012] Vincent assisted to the 10th International Plant Molecular Biology Congress in Jeju Island (Korea) and gave a talk on "Transcriptome changes associated to oil accumulation in oil palm (Elaeis guineensis Jacq.) mesocarp".


[October 25-26, 2012] The lipidomic plateform organized the "journée lipidomique" in Bordeaux. This event gathered 80 people (left picture).  Very fruitful discussions !   This was linked with the first meeting of the MetaboHUB project (right picture).



[October 22, 2012] PC, PS, PE, PI, PA... phospholipidomic in Bordeaux. Eric and Sophie (now  post doc in Shevchenko's lab, Dresden) are co-authors of a paper published in Analytical and Bioanalytical Chemistry with Corine Buré and JM Schmitter. Reversed phase and normal phase liquid chromatography, coupled to mass spectrometry, are compared to shotgun analysis in order to determine in a single run the yeast phospholipidome.

[October 19th, 2012] Laetitia came back from the GERLI conference in Paris
where she presented a poster about quantification and regiolocalization of triacylglycerols.


[October 10, 2012] Please visit the new web site about the Extension group LEB Aquitaine Transfert.



[October 1, 2012] The LBM, with the lipidomic department of the Metabolome plateform, organizes a “Journée Lipidomique” in Bordeaux next October 26. Find the program and the registration form by clicking here. Everybody is welcome.

[October 1, 2012] The Surface Lipid Team is delighted to welcome Camille Delude as PhD student after her brilliant contest at the Bordeaux II Doctoral School!! Camille will work on suberin biosynthesis with Frédéric Domergue.

[September 7th, 2012] Invited by Sébastien, Sylvain Raffaele (LIPM, Toulouse) came to give us a beautiful seminar entitled "How filamentous plant pathogens render plants susceptible ?" and to discuss the future of our common projects. It was nice to see you again, Sylvain...



[September 4th, 2012] Why the plant raft protein REMORIN is called after the remora fish? Because this protein, albeit completely hydrophyllic, sticks to the plasma membrane rafts like the litlle fish to the sharks and boats!
You want to know how ? Have a look of our new work, published in Plant Physiology to get the answer... This work was done by Artemis in Sébastien's team, in collaboration with  membrane biophysist colleagues from Belgium together with our former post-doc Sylvain Raffaele.



[August 30, 2012] We welcome for 1,5 month in the LBM, Martìn Mecchia post-doc from Buenos Aeres in Alicia Zelada's laboratory. In the frame of Ecos-SUD project he will work on remorin and PVX in the plant raft team led by Sébastien.


[August 1, 2012] The LBM says Good bye and Good luck to Amélie Bernard. After a brilliant phD, Amélie leaves to her first post-doc in Ann Arbor, Michigan, USA, to work on autophagy in eukaryotic cells in the Daniel Klionsky's lab. We will miss you. Keep in touch.

[July 20, 2012] Please visit our new web page about the lipidomic platform of the LBM.


[July 19, 2012] Véronique from the LBM published with our neighbours of the INRA campus a Plant Cell paper on tomato cutin. This study analyzes the mechanism by which cutin is deposited. GDSL1, which belongs to the GDSL esterase/acylhydrolase family of plant proteins, was found to play a key role in cutin deposition during fruit cuticle development 
(link).

[July 18, 2012] Sébastien, Artemis and Véronique wrote a review on lipid rafts on plant, fungi, and animals in eLS. You will discover the history on raft domain research.

[July 16, 2012] Nice lipid meeting under the hot sun of Andalusia: 42ºC ! Eight people from the LBM attended the 2012 International Symposium on Plant Lipids (ISPL) in Sevilla. René chaired the session "Surface lipids/Extracellular lipid metabolism" and four of us gave a talk.



[July, 9 2012] Houda and Sébastien (left) came back from Grenoble where they gave two talks at the  "7ème Congrès des Jeunes Chercheurs de la Société Française de Biologie Végétale". It was the opportunity to meet our lipidist colleagues from Grenoble and Paris (right).



[July 5, 2012] After more than 15 years of investigations on the role of CER1 in wax biosynthesis, questions are finally answered thanks to the beautiful work of Amélie and Jérôme which have shown, with their collaborators, that CER1 acts synergistically with CER3 and a cytochrome b5 to catalyze the synthesis of VLC-alkanes. Have a look to these results published in The Plant Cell and untitled “Reconstitution of plant alkane biosynthesis in yeast demonstrates that Arabidopsis CER1 and CER3 are core components of a VLC-alkane synthesis complex” (link).
You can also read something about this work in the journal of the University and in the Journal of CNRS.



[June 22, 2012] Here it is !! the LBM moved to its new location in the INRA campus. The lab is brand new. Please visit us when you have the opportunity to come to Bordeaux. See you soon.


[June 13, 2012] Did you know that the plant cell wall constrains lateral diffusion of  plasma-membrane proteins?  You should check the last paper in PNAS done by Alexandre Martinière (who visited the LBM several month ago) in John Runion's Lab, Oxford Brookes University, UK. Sébastien, Lilly and Patrick from the LBM are co-authors of this beautiful work.



[June 13, 2012] The LBM is packing to moove to the "green campus" in INRA facilities (south of Bordeaux) to join our plant biologist colleagues. Keep up date the news !



[June 7, 2012] Alisa Nakkaew is a graduate student from the Prince of Songkla University in Thailand. She will stay for about 10 months in our laboratory and will work on the enzymes of fatty acid biosynthesis in oil palm with Vincent Arondel. Welcome in the LBM.

[June 7, 2012] Pr Thierry Berges, from Poitiers University, gave this morning in the LBM a very interesting seminar about the toxic effect of oleic acid in yeast, leading to mitochondrial disorders and apoptosis. Thierry was invited by Eric Testet to discuss lipid metabolism and  analysis thrue the lipidomic platform. http://www.cgfb.u-bordeaux2.fr/fr/metabolome

[June 5, 2012] Finaly, a review on the biosynthesis and functions of plant fatty alcohols became available!! Thanks to Frédéric Domergue and Owen Rowland !!!!
http://dx.doi.org/10.1016/j.plantsci.2012.05.002



[June 5, 2012] Freddy Boutrot,  Post Doctoral Scientist in the Dr Cyril Zipfel's team at the Sainsbury Laboratory came to the LBM to speak about the recognition of invading microorganisms by detecting conserved molecules referred to as PAMPs (pathogen-associated molecular patterns) by pattern recognition receptors (PRRs). Very interesting indeed !



[May 21, 2012] ABC of LCB: You don't have an HPLC-coupled to a fluorimetre or to an MS, but you want to analyse Long Chain Bases (LCB) ?
Check our method recently published in Analytical and Bioanalytical Chemistry (ABC ; DOI 10.1007/s00216-012-6060-1) using GC-MS quantification after the conversion of LCB in corresponding fatty aldehydes.



[May 9, 2012] Dr Pascale de Lonlay from the Hôpital Necker-enfants malades in Paris gave in the lab this morning a very interesting talk on the autosomal recessive LPIN1 mutations recently described as a novel cause of rhabdomyolysis in few families. This protein is likely links with disorder of the lipid metabolism. Dr de Lonlay was invited by Eric Testet to discuss a collaboration with the LBM and the lipidomic platform.


[May 9, 2012] I
an Pulsifer from Pr O. Rowland’s lab at Carleton University (Ottawa, Canada) is visiting us for 10 days. He is supported by a FCRF grant (France Canada Research Fund) obtained by F. Domergue and O. Rowland. Ian will express Arabidopsis proteins in E. coli and characterize the compounds resulting from their expression by GC-MS. Have fun in Bordeaux!!



[May 7, 2012] "Lipids of plant membrane rafts". This is the title of our recent invited review published in Progress in Lipid Research (http://dx.doi.org/10.1016/j.plipres.2012.04.001). As stated, this article wrote conjointly by members of the LBM team "Biomembranes and Membrane Lipids" and our collaborators from dijon (Lab PME) and Bordeaux (Lab CBMN) reviews the current knowledge and perspectives on phospholipids, sphingolipids and phytosterols in plant raft microdomains going from model membranes to vizualisation and cellular functions.




ANOUNCEMENT: After the successful "Patchy Prague 2010", comes now "Dijon Domains 2012" https://colloque.inra.fr/dijon_domains
This conference, which will be held in Dijon from 27 to 30 November 2012,  will be dedicated to the most advanced methodologies allowing the study of membrane sub-compartmentation, the latest exciting discoveries concerning the characterization of membrane organization, and the most recent evidences indicating a crucial role of this organization and its dynamics in signaling processes and cell physiology.
Very famous speakers accepted the invitation in Dijon like Kai Simons, Erwin London, Luis Bagatolli, Katharina Gaus, Didier Marguet, Bridget Wilson, Tobias Walther and will give plenary invited lectures

[April 18, 2012] We were glad to hear the talk of Federica Brandizzi from Michigan State University, USA (http://www.prl.msu.edu/Facultypages/Brandizzi.html) on news and views in the activities of the plant Endoplasmic Reticulum. Federica whose is a close collaborator of Patrick Moreau from the LBM, was invited by our lab and the SFR "Biologie Intégrative et Ecologie".
Thank you Fede', for the visit, see you soon.



[Avril 18, 2012]  The project « MetaboHub » which aims to coordinate metabolomic researches between labelled IBISA platforms in Bordeaux (Metabolome : INRA, Bordeaux University), Paris (MetabolomeIDF, Paris-Saclay, CEA and Pierre and Marie Curie University), 
Toulouse (MetaToul, Paul Sabatier University, INSA, INRA, CNRS and INSERM), and Clermont-Ferrand (Exploration du Métabolisme, INRA and Blaise Pascal University) has been funded. The lipidomic platform in which several members of the LBM are implicated will be largely involved in the future development of new MS/MS lipid methodologies.

 

[Avril 16, 2012] Since the 1st of January 2012, ADERA (Association pour le Développement de l'Enseignement et des Recherches auprès des universités, des centres de recherche et des entreprises d'Aquitaine) created a technology transfer unit leaned back on our UMR (http://www.adera.fr/spip.php?article32). Its name: LEB Aquitaine Transfert, LEB stating for "Lipids, Environment and Biotechnologies". The main missions are to provide expertise and conduct research works in two distinct areas: 1/ Ecotoxicology (using the lipids as diagnostic tools), and 2/ Biotechnology of lipids of industrial interest.  Marina Le Guédard (Engineer-PhD) is the R&D responsible.



[March 30 , 2012]  Emmanuelle Bayer, member of the raft team at the LBM, has recently published with her former group in Bern Switzerland, headed by R. Smith and C. Kuhlemeier a paper published in Science. This work shows for the first time that mechanical signals are not just passive readouts of gene action but can feed back on morphogenesis.




[March 6-7 , 2012] Laetitia Fouillen came back from the 2nd Graz Lipid Mass Spec Meeting where she had the chance to learn and talk with lipid mass spec specialists. She presented a poster on the variability of fatty acid and TAGs compositions in palm oil using lipidomics.


[Mars 1, 2012] The LBM welcome several students : two M2 students,
Camille Delude who is working with Fred Domergue in the wax team and Etienne Legrand who is working on palm oil synthesis with Vincent Arondel; two M1 students, Simon Vanderliden with Claire Bréhelin and Julien Gronnier with Jérôme Joubès.

[February 6, 2012]
We welcome in the lab Guillaume Bouyssou, a new assistant engineer who will work for 2 years on the ANR project ACETOTRYP lead by Fréderic Bringaud (RMSB, Bordeaux), Jean-Charles Portais (INSA, Toulouse) and Patrick Moreau at the LBM.

[February 2, 2012]
Laetitia Fouillen (left) from the LBM represented the lipidome department of the Metabolome plateform at the “journée utilisateur” of the Functional Genomic Center of Bordeaux (http://www.cgfb.u-bordeaux2.fr/). Tristan Richard (left) et Catherine Deborde (right) are other members of the Metabolome platform.




The LBM wishes you a Happy New Year 2012




[January 9, 2011 ] The wax team decided to work on a new endemic species found in the lab. Good luck Amélie. Thanks god we are moving soon !!



[January 5, 2011 ] Yohann Boutte arrives in the lab as CR-CNRS in Patrick Moreau's team, after 6 years in Umeå, Sweeden, as postdoc in Markus Grebe
and then Rishi Bhalerao labs. We wish you all the best for your installation in Bordeaux and your experiments.
                                                                                 

[December 21, 2011]
The LBM is happy to congratulate our former post-doc Sylvain Raffaele, admitted CR1 INRA to work on Sclerotina / Arabdopsis at the LIPM in Toulouse with Dominique Roby and Yves Marco. Good luck for your future Sylvain. We were happy to have you two years in our lab.


[December 14, 2011] Alexandre Martinière, post-doc in the group of John Runion at Oxford Brookes UK, now in post-doc in  the "Intracellular Dynamic of Membrane Proteins" team led by Nadine Paris in Montpellier, France, came to the lab to give a seminar on lateral diffusion of plant membrane proteins, and the role of cytoskeleton and cell wall.


[December 9, 2011]  
Amélie Bernard get today her phD diploma in the wax team ! congrats.

[November 10,  2011]
Rémi Zallot get today his phD diploma in the lipase team ! Congratulation

[November 22, 2011] The famous “coffee NDR1” paper finally got accepted into BMC Plant Biology upon a long-lasting review process. Congratulations to all authors… and think of it when you have a coffee break! "Identification and characterization of the Non-race specific Disease Resistance 1 (NDR1) orthologous protein in coffee". Cacas JL, Petitot AS, Bernier L, Estevan J, Conejero G, Mongrand S, Fernandez D. BMC Plant Biol, Oct 24;11:144.

[November 21, 2011] The evaluation of the toxic effects of pollutants is difficult to grasp in a real and complex environment. We recently evidenced using Lactuca serriola that plant leaf fatty acid composition measured in situ would provide an early indication of a plant’s exposure to metal and could be used as a reliable tool to facilitate or strengthen the diagnosis of soil contamination.
Le Guédard et al (2012). Env. Exp. Bot,76, 54-59.


We recently characterized a new lyso-PA acyltransferase regulating the amount of triglycerides (TG) in S. cerevisiae. Using a lipidomic approach (HPTLC-GC; MS/MS) we demonstrated that this enzyme is responsible for the incorporation of oleic acid both in phospholipids and TG, whereas quantitatively only TG were decreased in mutant cells. In addition we showed by immunoblot, proteomic and confocal studies that this enzyme is mainly located in ER membranes and in lipid droplets during the exponential and the stationary phases respectively.
Ayciriex et al. (2012) Mol Biol Cell, in press



[November 17 and 18, 2011] We are hosting Drs Martine Devic and Thomas Roscoe from the Epigenetic Regulation and Seed Development Group, IRD, Montpellier. They did experiments to determine the lipid composition changes of different mutants of Arabidopsis thaliana and presented their results.


[September, 2011] Welcome to Agnès Chevin the new administrator of the lab.

[November 2, 2011] ICON strikes again!!! Collaboration between labs from Sweden, Canada, Australia and the LBM, all partners of the large collaborative project ICON (http://icon.slu.se/ICON/index.htm), resulted in the in vitro functional characterization of the plastidial fatty acid reductase AtFAR6. Have a look at its unexpected catalytic capacities in our 
"Publications" section.!!!


[October 21, 2011] Do not forget two importante dates for the lab, Rémi from the "Storage lipid metabolism" team will defend his thesis Thursday November 10th and Amélie  from the "wax" team will defend her thesis Friday December 9th.


[October 15, 2011]  
After a master 2 on plasmodemata with Emmanuelle Bayer last year, Houda Nacir begins this month her thesis with Claire Brehelin on plastoglobules, granted by the Conseil Régional d'Aquitaine, FRANCE.



[October 14, 2011] Good bye Jean Luc, After 2 years at the LBM and a successful post-doc, you leave to Dijon to work on defence signalling. All the best !!!



[October 1, 2011] Sébastien Mongrand has been nominated at the section 28 "Biologie végétale intégrative" of the comité national. http://www.cnrs.fr/comitenational/sections/section28.htm

[September 27, 2011] Rene Lessire was in Rotterdam, as a member of the scientific committee of the 9th EuroFed Lipid Congress “Oil, Fats and Lipids for a Healthy and Sustainable World” with more than 600 participants,150 lectures and 250 posters. René was chairman of the “Oilseeds, Plant breeding, Plant lipids” session and he presented a lecture on Arabidopsis CER1, the function of  which has been recently elucidated by Amélie Bernard and her collegues.
More information at: http://www.eurofedlipid.org/meetings/archive/rotterdam/index.htm


 During the coffee break René with A. Abbadi (middle) Lembke, and F. Spener (Graz University) (left)

[September 22, 2011] Patrick Moreau came back from The Society for Experimental Biology SEB meeting « Exocytosis in animals, fungi and plants »   organized in London by Viktor Zarsky (University of Praha), and where he gave a conference on “Links between lipid biosynthesis, organelle morphology and protein trafficking in plant secretory pathways”. This meeting gave a large part to plant research and exciting comparisons were discussed between plant cells and other eukaryotic models. This SEB symposium was also the opportunity to estimate that plant fields have reached similar levels of excellence than their mammal and yeast counterparts. The program of the symposium can be obtained at the SEB meeting site (http://www.sebiology.org/meetings/Exocytosis/index.html).




[September 12,  2011] Check our new review on Plant Rafts in Current Opinion in Plant Biology by the raft team (Sébastien, Artemis and Emmanuelle) together with our collaborators from Dijon, Françoise Simon-Plas and Patricia Gerbeau-Pissot. We discuss a working model of the putative involvement of rafts in the activation of RbohD after elicitation by PAMP.

This article is now published online at: http://dx.doi.org/10.1016/j.pbi.2011.08.003
Keep also track of lab updates publication with the "Activités" section.



[September 8, 2011] Have  a look at our last publication in PNAS, written with our swiss friends from Neuchâtel co-written by Claire Bréhélin from the LBM, about the plastoglobule localized enzyme AtNDC1 (a type II NADPH quinone oxidoreductase) and its role in plastoquinone oxidoreduction and prenylquinone metabolism. http://www.pnas.org/content/108/34/14354.long
Beside, all you always dreamt to know about Arabidopsis plastoglobule preparation is explained by Claire and her colleagues in this new chapter of Methods in Molecular Biology.
Besagni et al., " Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies" http://www.springerlink.com/content/r4l7803j80802744/#section=947110&page=1



[August 7, 2011] Viva Argentina !!! Owing to the ECOS-SUD France-Argentina project, Sébastien spent two weeks and Artemis four weeks at the University of Buenos Aires (top left picture). Our collaborator Alicia Zelada welcomed us to work on PVX propagation and the involvement of the plant raft protein REMORIN (bottom left picture, from left to right: Maria BINAGHI, Sébastien MONGRAND, Alicia ZELADA and Artemis PERRAKI).
We took the opportunity to visit Iguaçu Falls and the city of Buenos Aires and its strange baobabs…



[July 18, 2011]
We're back from Gdansk in Poland where the 5th european Symposium on Plant Lipids took place. As always, the congress was very interesting, we met lot of colleagues and several people from the lab had the opportunities to present their work (Amélie, Vincent, Fred, Rémi and Jérôme).


                  

[June 27, 2011]
Jean-Jacques Bessoule et Marina Le-Guedard came back from the island of Skiathos in Greece. Lucky you ! They attended the Third International CEMPE (Conference on Environmental Management , Engineering, Planning and Economics)  and SECOTOX (Society of Ecotoxicology and Environmental Safety and its Regional Sections) conferences.  An utmost interested congress dedicated to environment and ecotoxicology.

       

[June 28, 2011]
Our Laboratory (Oil group led by V. Arondel), in collaboration with John Ohlrogge's laboratory (MSU, USA) and two African scientists, has given a major contribution to a carbon partitioning study in palm mesocarps, which is now out in PNAS. Aside from its fundamental interest, this work opens the way to genetic improvement of oil palm, a major crop that grows almost exclusively in developing countries, with probable important long-term impact on development of these countries' agriculture.
Bourgis et al., 2011
[see also the CNRS press communication on the web: http://www2.cnrs.fr/presse/communique/2236.htm]
[see also:  http://www.techno-science.net/?onglet=news&news=9396]


 

[June 9, 2011]
The research led by J. Malek in Qatar and involving worldwide collaborations including our lab (oil team led by Vincent Arondel) on the draft genome of date palm makes the cover of Nature Biotechnology. This work has important implications for improvement of the crop with impact on sustainable agriculture in developing countries.



[May 30th, 2011] The new review written by Denis Coulon and his colleagues from the "Biogenèse et Homéostasie membranaire" team on N-acylphosphatidylethanolamines NAPE, not just precursors of  N-acylethanolamines (NAE) is out in Biochimie. Have a look !


[May 27th, 2011] Owen Rowland who is associate professor at Carleton University in Ottawa is coming from Canada for 2 weeks. He is supported by a France Canada Research Fund (FCRF) that he obtained in collaboration with Frédéric Domergue.


[May 17th, 2011] The collaborative paper of the two Patricks : Patrick Moreau from LBM and Patrick Legembre from University of Rennes, IRSET (Institut de Recherche en Santé, Environnement et Travail) about the  the oncogenic PI3K/Akt signalling pathway is out in European journal of Immunology

Pizon et al., PI3K/AKT inhibition elicits actin-independent and CD-95-dependent apoptic signalling through localisation of CD95 into lipid rafts.


[May 5th, 2011]
We are hosting Reinhard Jetter who is  professor at the University of British Columbia in Canada for one month in the lab. He is supported by a France Canada Research Fund (FCRF) that he obtained in collaboration with the people of the wax team.


[April 8th, 2011] The BSM paper of our belgium colleagues is out on line. The additional news are that PNAS Editorial Board selected our suggestion image for the cover of the print issue on the 19th April.
Babiychuk et al., Plastid gene expression and plant development require a plastidic protein of the mitochondrial transcription termination factor family.


Pictured is an Arabidopsis plant that is a functional mosaic produced by the controlled CRE/loxP recombination at an engineered BSM gene locus. 

[March 30th, 2011], the lab thanks Stéphane Blanc from INRA, Montpellier, and Christiane Laurière from ISV/CNRS, Paris for their participation of the mid-term PhD comitee of Artemis Perraki.


[March 30th, 2011] We are hosting Dr Christiane Lauriere from the Institute of Plant Sciences , Gif-sur-Yvette. She will talk about "Mechanisms of regulation of the SnRK2 kinase in osmotic signalling".



[March, 25th 2011]. Coming for the Rehearsal of the CNRS competition, we listen to Yohann Boutté' post-doc stories in Umeå Plant Science Centre, Sweeden, on phytosterols and sphingolipids and the establisment of cell polarity. Good luck Yohann for the competition !


[March 21st, 2011] We are hosting Maria Binaghi from the University of Buenos Aeres, Argentina, for three months in the lab. She is supported by a short EMBO fellowship and will work on REMORIN and PVX virus propagation. Good luck for the experiments and enjoy Bordeaux.



[March 15th, 2011] Eric Maréchal, Laboratoire de Physiologie Cellulaire Végétale, CEA, Grenoble, France, stopped by the lab and presented a talk on the chemical inhibition of galactolipid synthesis by molecules competing diacylglycerol.

[March 8th, 2011] The paper of Brice Bourdenx, Amélie Bernard, Stéphanie Pascal, René Lessire, Fred Domergue, Jérome Joubès on the role of CER1 protein in Arabidopsis is accepted in Plant Physiology. Congrats !!!

[March 4th, 2011] We are hosting Jean-Luc Montillet from the CEA at Cadarache. He gave a great talk on the role of oxylipin in plant physiology. Great discussions!

[February 5th,  2011]. Welcome Back Amélie Bernard and Sébastien Mongrand from the Gordon Conf’ on Plant Lipids: Structure, Metabolism & Function held in Galveston Texas. They even met G W Bush in Houston, as a statue…

[January 17th, 2011] Our new review on the links between lipid homeostasis, organelle morphodynamics and protein trafficking in eukaryotic and plant secretory pathways by Patrick Moreau, Valérie Wattelet-Boyer and Su Melser is out in Plant Cell Reports,

[January 7th, 2011] Our paper on the Identification of a putative triacylglycerol lipase from papaya latex by functional proteomics by Vincent Arondel V. is out in FEBS J.

[January 3rd, 2011] Welcome to Laetitia Fouillen, the new engineer of the lipidomic platform. Good luck for developing this important tool in the lab.

[December, 2011] The last review on plant membrane rafts by Sébastien Mongrand, Emmanuelle Bayer and our colleague form Dijon makes the cover of Trends in plant Science. Nice pic !




[October, 2010] Good Bye to Sophie Ayciriex, phD, she leaves the lab for her first post-doc in the laboratory of Olivier Laprévote in Paris. Good luck Sophie !!! All the best, and keep in touch

[October, 2010] Welcome to Didier Thoraval, Francois Doignon and Annie Claveres, a new team working on yeast in the lab.

[11 oct, 2010] We're back from BIARRITZ France where most of the lab attended the GERLI lipid meeting. Good science but also time to catch up with colleagues and friends

[11 oct, 2010] From the leaf to the lipid bilayer, through DNA helix… a story of membrane biogenesis. Here is our new LBM logo. The graphic designer is Sébastien de Royère from Bordeaux. Have a look on his web site. http://www.royeredubus.com/site.html.

[September, 1st 2010] Véronique Germain, assistant professor at the university of Bordeaux, joins the lab to work on the plant protein Remorin and viruses. She teaches plant science. Welcome in the lab Véro !!!

[1 june, 2010] Welcome Magalie Salmon, our new tech’, our first technician coming from the hospital world.