Université de
Bâtiment A3
Bordeaux Aquitaine
71 Avenue Edouard Bourlaux
CS 20032
33140 Villenave d'Ornon

Tél: 00 33 5 57 12 25 89
Fax: 00 33 5 57 12 26 44

Directeur : Dr Jean-Jacques Bessoule
English version
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Notre activité de Recherche


LEB Aquitaine Transfert



Dernière mise à jour : 14 Novembre 2016
Responsable éditorial: Jean-Jacques Bessoule
Responsable Com: Sébastien Mongrand
Jérôme Joubès


[2016, October 3] The LBM welcomes two new PhD students: Adiilah Mamode Cassim, under the supervision of Lilly on GIPCs, and Anne-Flore Deroubaix, with Véronique on REMORIN.
May your thesis be a success, ladies ! 

[2016, October 3] Marie Brault was recruited by the LBM for a one year assistant engineer position to work on plasmodesmata in the context of  the ANR project CONNECT coordinated by Manue.
All the best, Marie

[2016, October 3] To orient their growth plants target some proteins to one side of the cell. Valérie, Nicolas, Jérôme, Fred, Sébastien, Patrick and Yohann now reveal in a study published in Nature Communications that cells employ the peculiar lipid composition of one intracellular central hub called trans-Golgi Network (TGN), a post-Golgi compartment that act as a sorting station, to direct the auxin efflux carrier PIN2 towards the apical plasma membrane domain of epidermal roots cells. This work shows that the length of the acyl chain of sphingolipids is critical and points out that lipids cannot be considered anymore as simple bricks of membranes but should rather be viewed as “smart materials” empowering functional properties to membranes and the ability for multicellular organism to quickly adapt to environmental changes.   

A press release is available at:

Enrichment of hydroxylated C24- and C26-acyl-chain sphingolipids mediates PIN2 apical sorting attrans-Golgi network subdomains
Valérie Wattelet-Boyer, Lysiane Brocard, Kristoffer Jonsson, Nicolas Esnay, Jérôme Joubès,Frédéric Domergue, Sébastien Mongrand, Natasha Raikhel, Rishikesh P. Bhalerao, Patrick Moreau& Yohann Boutté
Nature Communications. 7. Article number:12788 doi:10.1038/ncomms12788

[2016, October 3] The LBM welcomes a new researcher with a CNRS position ! After her thesis with Jérôme at the LBM, and a post-doc in Daniel Klionsky's lab, University of Michigan USA, Amélie Bernard will develop a new project  at the LBM on autophagy in plants.
All the best for your research! 

[2016, October 3] Christophe Rocher (Ingénieur d'études at the University of Bordeaux) just arrived in the LBM. Welcome !

[September, 19 2016] Aimez vous les lipides de plantes?
Camille Delude , Jérôme Joubès,  and Frédéric Domergue of the LBM contribute to a chapter entitled "Plant Surface Lipids and Epidermis Development" in the book "Lipids in Plant and Algae Development" Yuki Nakamura • Yonghua Li-Beisson Editors.

[September 5, 2016] The LBM welcomes Sylvain Kenan on MetaboHub contract for 6 months to develop sphingolipidomic analysis. Good luck in your job !

[September 2, 2016] Yohann and Patrick have organized the 19th ENPER (European Network for Plant Endomembrane Research) meeting in Bordeaux from August 30 to September 02 - 2016.

ENPER is the first and oldest international conference on plant membranes research (created in 1996 by Pr. David Robinson). This year, about 120 scientists gathered essentially from Europe but also from around the world (North America, South America and Asia) participated to this meeting at the IBIS in Bordeaux.
Themes covered studies on the elements of the trafficking machineries in secretion, endocytosis and recycling pathways, and function and dynamics of the endomembranes and plasma membrane in cell polarity and plant development. According to the original philosophy of this meeting, time to speak was essentially given to young researchers (PhD students and Post-docs). The discussions during the meeting were rich and intense.
This meeting was also the opportunity of our community to celebrate the fantastic career of Pr. Natasha Raikhel.
The meeting was a great success, thanks to all the speakers and participants! 

[July 3-8, 2016] Jerome, Yijun, Vincent and Fred came back from Goettigen in Germany where they attended the 22th International Symposium on Plant Lipids organized by Ivo Feussner. Jerome gave a talk on the function of CER1 proteins in the biosynthesis of cuticular alkanes in Arabidopsis.

[July 4-7, 2016] Laetitia and Patrick participated to the annual meeting of the SEB (Society of Experimental Biology) in Brighton, UK on 4 to 7 of July. Patrick gave a talk entitled "Lipid physical properties, membrane dynamics and transport, and lysophospholipid acyltransferases at the ER-Golgi interface in plant cells". Laetitia and Patrick organised a workshop on Plant ER membrane lipids: analysis, composition and functional aspects.

[June 6, 2016] Laetitia contributed to a publication in PNAS on the Fragile X Syndrome pathology. This work supports a model where FMRP (Fragile X Mental Retardation Protein), by controlling the translation of the DiacylGlycerol Kinase kappa (DGKκ), indirectly controls synaptic protein translation and membrane properties by impacting lipid signaling in dendritic spine.

Tabet R., Moutin E., Becker J.A., Heintz D., Fouillen L., Flatter E., Krężel W., Alunni V., Koebel P., Dembélé D., Tassone F., Bardoni B., Mandel J.L., Vitale N., Muller D., Le Merrer J., Moine H. (2016) Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase activity in neurons. Proc Natl Acad Sci U S A, in press

May 30, 2016] Camille, Laetitia, Stéphanie, Jérôme and Fred, in collaboration with Owen Rowland (Carleton University, Canada) and Dylan Kosma (University of Reno, USA), published a paper showing that most of the fatty alcohols not covalently linked to the suberin polymer are in the form of alkyl hydroxycinnamates (AHCs), with alkyl caffeates predominating. Such compounds are not restricted to the periderm of mature roots, but are also present in the endodermis of younger roots, where they are not extracted by rapid dipping in chloroform.
Delude C., Fouillen L., Bhar P., Cardinal M.-J., Pascal S., Santos P., Kosma D.K., Joubès J., Rowland O. and Domergue F. (2016) Primary fatty alcohols are major components of suberized root tissues of Arabidopsis in the form of alkyl hydroxycinnamates. Plant Physiol., doi:10.1104/pp.16.00834

[May 30, 2016] The ANR projet blanc PANACEA " PlANt plAsma membrane lipid signalisation in biotiC interactions " coordinated by Sébastien in collaboration with three laboratories in Dijon (UMR PME Plante-Microbe-Environnment), Paris (UMR PCMP Physiologie Cellulaire et Moléculaire des Plantes) and Bordeaux (CBMN, UMR Laboratoire de Chimie Biologie des Membranes et NanoObjets) ends with a publication  in  Plant Cell & Environment on the role of Diacylglycerol kinases in cryptogein signaling.
Thank you to everybody for these fruitfull collaborations and publications.

Cacas JL, Gerbeau-Pissot P, Fromentin J, Cantrel C, Thomas D, Emmanuelle J, Kalachova T, Mongrand S, Simon-Plas F & Ruelland E (2016) Plant Diacylglycerol kinases activate tobacco NADPH oxidase dependent oxidative burst in response to the PAMP cryptogein. Plant Cell & Environment, in press

[May 20, 2016] The LBM invited Eric Hosy (Institut Interdisciplinaire de Neurosciences, CNRS/Université de Bordeaux) through the invitation program of the FR BIE for a very interesting seminar entitled "How single particle based super-resolution techniques succeed to improve our vision of molecular dynamic organization of the cell". Thank you for the nice discussion on super-resolution microscopy.

[May 13, 2016] Juliette Jouhet from the Laboratoire de Physiologie Cellulaire et Végétale in Grenoble gave a seminar invited by Florence Corellou LBM and the FR BIE on "AtMic60 is involved in plant mitochondria lipid trafficking and is part of a large complex". Than you for the discussions on membrane contact sites.

[April 20, 2016]
Jerome and Fred, in collaboration with the team of Christophe Rothan from BFP unit in Bordeaux, participated in the characterization of a new tomato (Solanum lycopersicum) cutin mutant. The fruit cuticle thickness, composition and properties were altered in the gpat6 mutant. In vitro assays with recombinant proteins produced in yeast showed that a point mutation adjacent to the active site of the GPAT6 enzyme completely abolished its acyltransferase activity.


J. Petit, C. Bres, J.-P. Mauxion, F. Wong Jun Tai, L.B.B. Martin, E.A. Fich, J. Joubès, J.K.C. Rose, F. Domergue and C. Rothan (2016) The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis. Plant Physiol., doi:10.1104/pp.16.00409.

[April 6, 2016] Camille, Jérôme and Fred, in collaboration with Steven Moussu and Gwyneth Ingram from the RDP lab in Lyon, published a review,
in the book "Lipids in Plant and Algae Development" edited by Y. Nakamura and Y. Li-Beisson, on the relationship between plant surface lipids and epidermis development and the difficultly of functionally separate epidermal cell fate specification from cuticle biogenesis.  

Delude C.
, Moussu S., Joubès J., Ingram G. and Domergue F. (2016) Plant surface lipids and epidermis development. In "Lipids in Plant and Algae Development", "Subcellular Biochemistry" vol. 86, Nakamura Y. and Li-Beisson Y. (eds), Springer International Publishing Switzerland, pp 287-313.

[March 24, 2016] Yohann and Magali, in collaboration with the team of Shuzhen Men (College of life sciences, Nankai University, China), contributed to a publication in Plant Physiology on the functional role of an enzyme of the sterol biosynthesis pathway, the sterol 4α-methyl oxidase2 protein (SMO2)  in regulation of embryogenesis in Arabidopsis through auxin accumulation and PIN1 expression defect. This work strengthens the idea that precursors of bulk sterols are involved in plant development and that a correct balance between precursors and bulk sterols is critical for plants.

Xia Zhang, Shuangli Sun, Xiang Nie, Yohann Boutté, Magali Grison, Panpan Li, Susu Kuang, Shuzhen Men (2016). The Arabidopsis Sterol 4α-Methyl Oxidase2 Family is esssential for Auxin-Regulated Embryonic Development. Plant Physiology, in press.

[March 11, 2016] Invited by Sébastien Mongrand throught the FR BIE,  Sophie Layé (Nutrition and Integrative Neurobiology, INRA Bordeaux University) - Lauréate du Laurier Défi Scientifique 2015 - gave a very interesting seminar on "Nutritional Omega 3, what role in the brain?"

[March 10, 2016] Phytolongins are a new family of non-SNAREs longins in plant cells. Four proteins have been identified in Arabidopsis (Phyl 1.1, 1.2, 2.1 and 2.2) and their subcellular localization and trafficking has been determined in this work published by our team (Valerie Wattelet-Boyer, Guillaume Bouyssou, Claireline Marais, Yohann Boutté and Patrick Moreau ) in Journal of Experimental Botany with the strong collaboration of Carine de Marcos Lousa (University of Leeds-Becket) and Francesco Filippini (University of Padova).
In this study, we found that the four phytolongins are distributed along the secretory pathway, Phyl2.1 and Phyl2.2 were strictly located at the ER network, Phyl1.2 at the Golgi bodies and Phyl1.1 was mainly associated to the plasma membrane and partially to post-Golgi compartments. Our results show that Phyl1.1 export from the ER depends on the GTPase Sar1, the Sar1 guanine nucleotide exchange factor Sec12, and the SNAREs Sec22 and Memb11. In addition, we have identified the Y48F49 motif as being critical for the escape of Phyl1.1 from the ER. Our results provide the first characterization of the sub-cellular localization of this new class of longins called phytolongins and discuss their potential roles in membrane structure and trafficking.

Carine de Marcos Lousa, Eric Soubeyrand, Paolo Bolognese, Valerie Wattelet-Boyer, Guillaume Bouyssou, Claireline Marais, Yohann Boutté, Francesco Filippini and Patrick Moreau.
Subcellular localization and trafficking of phytolongins (non-SNARE longins) in the plant secretory pathway.
J. Exp. Bot. 2016, in press.

[January 11-12, 2016] Antoine Loquet and Erick Dufourc organised the Aquitaine-Liège Symposium on "Plant lipids, membranes and NMR" at IECB with LBM teams and biophysicists from Gembloux working on membranes: two very intereting days. Thank you for the organisation.

[January 4, 2016] Three new master 2 students enter the LBM: Anne-Flore Deroubaix with Véronique Germain, Adiilah Mamode Cassim with Sébastien Mongrand, and Amazigh Hamaï with Vincent Arondel. WELCOME ABOARD

Happy New Year

[December 22, 2015] François Doignon and his team demonstrated that phosphoinositides containing stearic acid are involved in the control of intracellular trafficking and polarity of the actin cytoskeleton. For the first time, a new picture of actin cytoskeleton with bipolar structures is revealed in psi1Δ strains deficient for phosphoinositides with stearic acid and in mutants affected for bipolar budding pattern.

Doignon F., Laquel P., Testet E., Tuphile K., Fouillen L. and Bessoule J.J. (2016) Requirement of phosphoinositides containing stearic acid to control cell polarity. Molecular and Cellular Biology, in press

[December 15, 2015] Camille Delude
defended her Ph. D. thesis directed by Frederic Domergue on the mechanism of suberin monomer export and the relevance of fatty alcohols in suberin biochemistry. The comitee was constituted by Bénédicte Bakan from INRA Nantes, Fred Beaudoin from Rothamsted Research, Gwyneth Ingram from ENS Lyon and Dominique Rolin from INRA Bordeaux. Congrats Dr Delude !

[December 11, 2015] Valérie Wattelet-Boyer
succesfully defends this afternoon her EPHE diploma (École Pratique des Hautes Études) on the role of sphingolipids in the secretory pathway during Arabidopsis root gravitropism. Congratulations !

[October 25-30, 2015] Manue Bayer attented the 11th international congress of plant molecular biology in Igassu / Brasil. She gave a seminar on plasmodesmata. Of course, she took the opportunity to visit the beautiful falls (with our colleague from Bordeaux, Yves Gibon).

[November 13, 2015] Manue Bayer thrue the FR BIE invited Waltraud SCHULTZE (Universität Hohenheim, Stuttgart) to talk of "Definition and functional analysis of membrane microdomains in plants". Nice talk. Thank you for coming !

[November 8, 2015]
Godswill Ntsomboh is the recipient of a short term EMBO fellowship and he arrived in the laboratory on October 19. He comes from the Center for research on oil palm (CEREPAH) of La Dibamba in Cameroon. He will stay in the laboratory for 3 months, to analyse palm oil. Welcome Godswill!

[November 2, 2015] QUIZZ: What is the main sphingolipid on earth? 

Glycosyl Inositol Phosphoryl Ceramides (GIPCs), of course !
Discover in our recent publication in Plant Physiology entitled "Re-visiting plant plasma membrane lipids in tobacco: a focus on sphingolipids", the putative role in plant membrane biology of these interesting lipids.

Cacas J.L., Buré C., Grosjean K., Gerbeau-Pissot P., Lherminier J., Rombouts Y., Maes E., Bossard C., Gronnier J., Furt F., Fouillen L., Germain V., Bayer E., Cluzet S., Robert F., Schmitter J.M., Deleu M., Lins L., Simon-Plas F., Mongrand S. (2015) Re-visiting plant plasma membrane lipids in tobacco: a focus on sphingolipids. Plant Physiology, in press

[October 5, 2015]
Fred Domergue attended last week the 13th Euro Fed Lipid Congress in Florence (Italy), for which he organized and chaired together with Ignace De Bruyne (ID&A, Belgium) the plant lipid sessions. Fred also gave two oral presentations on projects from his team.

[October 1st, 2015] Two new PhD students arrive at the LBM: Paul GOUGUET with Sébastien Mongrand to work on the plant raft protein REMORIN, and Nicolas ESNAY with Yohann Boutté to work on Trans Golgi Network and the secretory pathway. All the best for your thesis.

[September 1st, 2015] The LBM is happy to welcome several new collaborators, a new engineer, Françoise IMMEL from Dijon; a new technician, Karine TUPHILE from Orsay, and one new post-doc Amelia GASTON-DAUZON in the team of Manue Bayer to work on plasmodesmata.  WELCOME TO LBM !

[August 26, 2015] If you are interested by SNAREs and GTPases in the plant secretory pathway, you are invited to read our recent paper dealing with the specific interaction of the Qb-SNARE Memb11 with the GTPase Arf1 in the Golgi apparatus of Arabidopsis which is published in the Journal of Experimental Botany.

"The Qb-SNARE Memb11 interacts specifically with Arf1 in the Golgi apparatus of Arabidopsis thaliana" by Marais C, Wattelet-Boyer V, Bouyssou G, Hocquellet A, Dupuy JW, Batailler B, Brocard L, Boutté Y, Maneta-Peyret L and  Moreau P.

[July 5-9, 2015]
Meanwhile, Vincent and Claire attented the 26th ICAR (International Conference on Arabidopsis Research) in Paris.

[July 6-8, 2015] Camille, Charlotte, Florence, Fred and Sébastien came back from Rothamsted. They attended the 7th European Symposium on Plant Lipids in the newly opened conference centre on site at Rothamsted Research, the oldest agricultural research institute in the world, based in Harpenden, Hertfordshire, UK.
They visited during the meeting the  phenotypage facility of the research center and the first open-field of GM plants in UK (Camelina producing fish oil); top pictures.

[June 17, 2015] Houda Nacir defended her Ph. D. thesis directed by Claire Brehelin on the lipid composition of plastoglobules. The comitee was constituted by Hubert Schaller, Jean-Luc Montillet, Justine Bertrand-Michel, François, Laetitia and Claire. Congrats Dr Houda !

[June 16-20, 2015] Jérôme and Fred gave seminars at the Plant Waxes workshop in Switzerland where they had deep discussions with the paleoclimatologist/geochimist community on isotope proxies in ancient sedimentary archives.

[May 28, 2015] Manue invited Bruno Humbel (head of the Electron Microscopy Facility of the University of Lausanne) for a seminar on the state of the art Correlative Microscopy.

[May 28, 2015] Bruno Humbel, Jens Tislner (University of St Andrews, UK) and Sylvain Trepout (Institut Curie, Paris) came for the mid-term comitee of William Nicolas. Thank you very for the very interesting discussions.

[April 24, 2015] Cyril Zipfel came from The Sainsbury Laboratory to give a seminar on "Connecting the dots: linking PAMP perception to receptor kinase-mediated immune outputs" invited by the LBM through the FR BIE.

[April 25, 2015] Niko Geldner came from the University of Lausanne to talk about "The endodermis - a tale of two cell types "

Both Cyril and Niko came for the mid-term comitee of Julien Gronnier. Thank you very for the very interesting discussions.

[March 30, 2015]
What is the lipid composition of plasmodesma-membrane lined channels ? This question was finally tackled by several members of the LBM in a recent publication in The Plant Cell. In collaboration with colleagues in Leeds (UK) and Bonn (Germany) we showed for the first time that membrane lipids are important for PD function: Sterols modulate PD targeting of GPI-anchored proteins and change callose-mediated cell-to-cell connectivity . 

Grison M.S., Brocard L., Fouillen L., Nicolas W., Wewer V., Dörmann P., Nacir H., Alfonso-Benitez Y., Claverol S., Germain V., Boutté Y., Mongrand S. & Bayer E.M. (2015) Lateral segregation of lipids at primary plasmodesmata defines plasmodesmal localisation of two GPI-anchored proteins in arabidopsis. The Plant Cell, in press

[March 30, 2015] Jean-Jacques in collaboration with Tom Roscoe and Martine Devic (IRD, UMR DIADE, Montpellier), contributed to a publication in Plant Cell Physiology on ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2 (LEC2), collectively the AFL, that are master regulators of seed maturation processes.
The  study examined the role of AFL in the production of seed reserves in Arabidopsis. Quantification  of seed reserves and cytological observations of afl  mutant embryos show that protein and lipid but not starch reserves are spatially regulated by AFL. Although AFL contribute to a common regulation of reserves, ABI3 exerts a quantitatively greater control over storage protein content whereas FUS3 controls lipid content to a greater extent. Whereas ABI3 controls the reserve content throughout the embryo, LEC2 and FUS3 regulate reserves in distinct embryonic territories
Roscoe et al (2015) Complementation of Seed Maturation Phenotypes by Ectopic Expression of ABSCISSIC ACID INSENSITIVE3, FUSCA3 and
LEAFY COTYLEDON2 in Arabidopsis. Plant Cell Physiol, in press

[February 25, 2015] Lilly and Patrick collaborated with the team of Alessandra Moscatelli (University of Milano) to characterize isolated membrane domains from pollen tubes of Nicotiana tabacum.

Moscatelli A, Gagliardi A, Maneta-Peyret L, Bini L, Stroppa N, Onelli E, Landi C, Scali M, Idilli AI, Moreau P. (2015) Characterisation of detergent-insoluble membranes in pollen tubes of Nicotiana tabacum (L.). Biol Open. 2015, doi: 10.1242/bio.201410249.

[February 2-6, 2015] Manue gave a seminar at the Gordon Conference on Plant Lipids, in Texas : Linking Plasmodesmata Membrane Lipid Composition and Cell-to-Cell Connectivity in Plants...and took the opportunity to visit the NASA's Space Center in Houston !
After working on plasmodesma, she plans to be an astronaut !

[January 19, 2015] Jérôme and the wax team members, in collaboration with the Team of Ljerka Kunst (UBC Vancouver), contributed to a publication in Plant Physiology on the functional characterization of the CER2-LIKE proteins and their importance for cuticle formation and pollen coat function.
Haslam T.M., Haslam R., Thoraval D., Pascal S., Delude C., Domergue F., Manas-Fernandez A., Beaudoin F., Napier J.A., Kunst L. and Joubès J. (2015). CER2-LIKE proteins have unique biochemical roles and physiological functions in very-long-chain fatty acid elongation.

cer2 cer2-like1 cer2-like2 pollen grain