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Plant lipid droplets from non-seed cells

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Group leader : Claire Bréhélin, researcher CNRS and Denis Coulon, associate professor


Claire Bréhélin
- 1999-2002 : Ph.-D. thesis at the laboratory « Génome et Développement des Plantes » of the University of Perpignan (France) : Characterization of cytosolic thioredoxin targets.
- 2003-2007 : Assistant professor at the Plant Physiology Laboratory of the University of Neuchâtel (Switzerland) : Protein composition and function of plastoglobules.
- 2008 - 2009 :CDD Research engineer in the Virology team of the INRA-Univ. of Bordeaux unit UMR1332 at Villenave d’Ornon (France) : Use of viral vectors to functionally validate the production of therapeutics in plants.
- Since 2009 : Chargé de Recherche 1ère classe au LBM

contact :
Phone : 00 33 5 57 12 25 39
E-mail : claire.brehelin@u-bordeaux.fr

Denis COULON obtained his PhD in Biotechnology and Food industries in 1997 at the National Polytechnic Institute of Lorraine and his HDR in 2006 at Bordeaux University. He worked as graduate assistant (1997-1998) at National Polytechnic Institute of Lorraine and as an assistant professor at the National School of Engineers on Agricultural and Food Industries of Nantes, France (1998). In 1999, he obtained a full position of associate professor at the ENSTBB, a graduate School of Biotechnology and Bioprocessing at Bordeaux and joined the LBM in 2005.

contact :
Phone : 00 33 5 57 12 25 67
E-mail : denis.coulon@bordeaux-inp.fr

Group members : JJ. Bessoule (DR), C. Bréhélin (CR), D. Coulon (MC), L. Fouillen (IR), K. Tuphile (TR), A. Hamaï (IE-Vitapalm)
















Abstract
In plant, seeds contain mainly triacylglycerols as reserve lipids, stored in cytosolic droplets whereas in leaves, most abundant lipids (phospho- and galactolipids) are components of cell membranes. In response to several stresses, membrane lipids are transformed into reserve lipids and stored as plastoglobules in chloroplasts or as lipid droplets in the cytosol. While seed lipid droplets attract much attention, these structures, as well as plastoglobules, are poorly studied in leaves. In particular their composition and mechanisms related to their synthesis remain largely unknown.



Micrograph of an Arabidopsis thaliana leaf cell showing plastoglobules inside chloroplast and one lipid droplet (LD) in the cytosol.

Our research team aims to describe precisely these structures and the molecular basis involved in their formation in leaves. For this purpose, we develop approaches of reverse genetics, proteomics, lipidomics, and microscopy (photonic and electronic).

Collaborators :
Brocard Lysiane , PIV, Bordeaux
Claverol Stéphane, CGFB, Bordeaux
Rothan Christophe, UMR1332INRA, Bordeaux
Immel F, UMR5200, Bordeaux


Former members :
Houda Nacir PhD 2011-2014
Nicolas Esnay M2 2014
Pauline Mure M1 2015
Lucie Combes-Soia M2 2013