Group leaders: Laetitia Fouillen and Sébastien Mongrand

Laetitia Fouillen is Research engineer (IR) at the CNRS. Initially trained as a chemist at the University of Paris-Sud and Strasbourg (France), she received in 2009 a Ph.D. in Analytical Chemistry from University of Strasbourg (LSMBO, Dr A. Van Dorsselaer). Then, she joined the Department of Physiology at the University of Fribourg (Switzerland) to work as a postdoctoral fellow on neuropeptidomics. In 2011, she moved to Bordeaux and now led the lipidomic platform in the LBM. ( ORCID link ).

Sebastien Mongrand is DR at the CNRS. He defended his Ph.D. in 1998 at
the LBM in chloroplastic lipid biosynthesis. After a three years post
doc at the University of Rockefeller (New-York, USA) working on ABA
signaling pathway, he was recruited CNRS in 2002. He is now co-leading
the “Lipids, Membrane Compartmentalization, Trafficking and
Morphogenesis in Plant and Yeast” with P. Moreau. He also works in the
platform as scientific co-leader.

Team members: P. van Delft (AI), Y. Boutté (DR), D. Bahammou (PhD)

Activity overview:

Since 2005, the LBM created a lipidomic platform belonging to the metabolome platform of Bordeaux of CGFB – Functional Genomic Center of Bordeaux and French national infrastructure of metabolomics and fluxomics MetaboHub.

The platform brings together instrumentations and expertise dedicated to the analysis of the lipidome and the study of lipids in their diversity. It is dedicated to the study of many organisms, from plants to animals and yeast.
Metabolome platform provides support for research in biology, environmental physiology, functional genomics and genetics in the context of regional, national and international collaborations. It is also used in the analysis of products derived from plants and other organisms. It is mainly used for the establishment and interpretation of lipidomic profiles, identification and structural analysis of lipids and functional analysis of lipid metabolism.

The platform provides identification, characterization or quantification (absolute or relative) of lipid:
– Quantification by densitometry of class of lipids
– Identification and quantification of fatty acid (long chain, hydroxylated…) composition, waxes, cutines and suberins components, long chain based (LCB)
– Relative quantification of molecular species of phospholipids (PC, PE, PG, PS, PI and PIPx), neutral lipid (TAG, DAG, steryl ester…).
– Regiolocalization for TAG molecules
– Quantification of Alkyl HydroxyCinnamates (alkyl ferulate, alkyl coumarate and alkyl caffeate)
We adapted the lipid extraction and analyses to the desired materials.

Analytical development

We are currently developing an analytical method for plant shingolipids, galactolipids and phosphoinositides by LC-MS.

Equipment
For our lipidomics analyses, we used different technologies: TLC, GC-FID, GC-MS and LC-MS. Reservation

Our analytical equipment can be used after specific training

– Mamode Cassim, A., Navon, Y., Gao, Y., Decossas, M., Fouillen, L., Grelard, A., Nagano, M., Lambert, O., Bahammou, D., Van Delft, P., Maneta-Peyret, L., Simon-Plas, F., Heux, L., Jean, B., Fragneto, G., Mortimer, J. C., Deleu, M., Lins, L., & Mongrand, S. (2021). Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation. J Biol Chem, 100602.

– Ito, Y., Esnay, N., Platre, M. P., Wattelet-Boyer, V., Noack, L. C., Fougere, L., Menzel, W., Claverol, S., Fouillen, L., Moreau, P., Jaillais, Y., & Boutte, Y. (2021). Sphingolipids mediate polar sorting of PIN2 through phosphoinositide consumption at the trans-Golgi network. Nat Commun, 12(1), 4267.

– Platre, M. P., Bayle, V., Armengot, L., Bareille, J., Marques-Bueno, M. D. M., Creff, A., Maneta-Peyret, L., Fiche, J. B., Nollmann, M., Miege, C., Moreau, P., Martiniere, A., & Jaillais, Y. (2019). Developmental control of plant Rho GTPase nano-organization by the lipid phosphatidylserine. Science, 364(6435), 57-62.

– Delude, C., Fouillen, L., Bhar, P., Cardinal, M.-J., Pascal, S., Santos, P., Kosma, D. K., Joubès, J., Rowland, O., & Domergue, F.
(2016). Primary alcohols are major components of suberized root tissues of Arabidopsis in the form of alkyl hydroxycinnamates. Plant Physiology, 171, 1934-1950.

– Kassas, N., Fouillen, L., Gasman, S., & Vitale, N. (2021). A Lipidomics Approach to Measure Phosphatidic Acid Species in Subcellular Membrane Fractions Obtained from Cultured Cells. Bio Protoc, 11(12),e4066.

– Brocard, L., Immel, F., Coulon, D., Esnay, N., Tuphile, K., Pascal, S., Claverol, S., Fouillen, L., Bessoule, J. J., & Brehelin, C. (2017). Proteomic analysis of lipid droplets from arabidopsis aging leaves brings new insight into their biogenesis and functions. Frontiers in Plant Science, 8, 894.

• 2012 Mélanie Onofre (BTS)
• 2013 Alice Choury (L3)
• 2013 Lynda Bensalem (M1)
• 2014 Lucie Combes-Soia (M1), Lionnelle Belanga (BTS)
• 2015 Julie Pinelli (AI MetaboHub), Florian Payen (M2)
• 2016 Sylvain Kenan (AI MetaboHub)
• 2017 Salimata Diarrassouba (AI MetaboHub)
• 2019 Alice Bacon (M2)
• 2020 Adriana Dinot (BTS)
• 2021 Marine Bayrand (L3), Erwan Goulevant (BTS), Amélie Perez (M2)
• 2022 Manon Genva (Post-doc)