Responsible: Claire Bréhélin, Researcher CNRS and Denis Coulon, Professor Associate Bordeaux-INP

1999-2002 : Ph.-D. thesis at the laboratory « Génome et Développement des Plantes » of the University of Perpignan (France) : Characterization of cytosolic thioredoxin targets.

 

2003-2007 : Assistant professor at the Plant Physiology Laboratory of the University of Neuchâtel (Switzerland): Protein composition and function of plastoglobules.

 

2008 – 2009 :    CDD Research engineer in the Virology team of the INRA-Univ. of Bordeaux unit  UMR1332 at Villenave d’Ornon (France) : Use of viral vectors to functionally validate the production of therapeutics in plants.

 

Since 2009 : Researcher at LBM

Denis COULON obtained his PhD in Biotechnology and Food industries in 1997 at the National Polytechnic Institute of Lorraine and his HDR in 2006 at Bordeaux University. He worked as graduate assistant (1997-1998) at National Polytechnic Institute of Lorraine and as an assistant professor at the National School of Engineers on Agricultural and Food Industries of Nantes, France (1998). In 1999, he obtained a full position of associate professor at the ENSTBB, a graduate School of Biotechnology and Bioprocessing at Bordeaux and joined the LBM in 2005.

Group members:

Bessoule J.J. (DR), I. Bouchnak (Post-Doc)

Thematique:

In plant, seeds contain mainly triacylglycerols as reserve lipids, stored in cytosolic droplets whereas in leaves, most abundant lipids (phospho- and galactolipids) are components of cell membranes. In response to several stresses, membrane lipids are transformed into reserve lipids and stored as plastoglobules in chloroplasts or as lipid droplets in the cytosol. While seed lipid droplets attract much attention, these structures, as well as plastoglobules, are poorly studied in leaves. In particular their composition and mechanisms related to their synthesis remain largely unknown.

Key articles

• Bermúdez L, Del Pozo T, Silvestre Lira B, de Godoy F, Boos I, Romanò C, Previtali V, Almeida J, Bréhélin C, Asis R, et al (2018) A Tomato Tocopherol-Binding Protein Sheds Light on Intracellular α-Tocopherol Metabolism in Plants. Plant Cell Physiol 59: 2188–2203

• Brocard L, Immel F, Coulon D, Esnay N, Tuphile K, Pascal S, Claverol S, Fouillen L, Bessoule J-J, Bréhélin C(2017) Proteomic Analysis of Lipid Droplets from Arabidopsis Aging Leaves Brings New Insight into Their Biogenesis and Functions. Front Plant Sci. doi: 10.3389/fpls.2017.00894

• Burgos E, Luca MBD, Diouf I, Haro LA de, Albert E, Sauvage C, Tao ZJ, Bermudez L, Asís R, Nesi AN, et alValidated MAGIC and GWAS population mapping reveals the link between vitamin E content and natural variation in chorismate metabolism in tomato. Plant J. doi: 10/gh3mrf

• Coulon D, Brocard L, Tuphile K, Bréhélin C (2020) Arabidopsis LDIP protein locates at a confined area within the lipid droplet surface and favors lipid droplet formation. Biochimie 169: 29–40

• Degraeve-Guilbault C, Bréhélin C, Haslam RP, Sayanova O, Marie-Luce G, Jouhet J, Corellou F (2017) Glycerolipidome peculiarities of the smallest green picoeukaryote Ostreococcus tauri. Plant Physiol pp.01467.2016

• Gutbrod P, Reichert S, Gutbrod K, Hamai A, Bréhélin C, Ngando-Ebongue G, Dörmann P (2021) Fatty acid isoprenoid alcohol ester synthesis in fruits of the African Oil Palm (Elaeis guineensis). Phytochemistry 185: 112684

• Nacir H, Bréhélin C (2013) When proteomics reveals unsuspected roles: the plastoglobule example. Front Plant Sci 4: 114
• Sofer L, Cabanillas DG, Gayral M, Téplier R, Pouzoulet J, Ducousso M, Dufin L, Bréhélin C, Ziegler-Graff V, Brault V, et al (2017) Identification of host factors potentially involved in RTM-mediated resistance during potyvirus long distance movement. Arch Virol 162: 1855–1865

Collaborations:

Agyei-Dwarko Dan, CSIR-OPRI, Kade, Ghana.

Brocard Lysiane , PIV, Bordeaux

Carrari Fernando, Instituto Nacional de Tecnologia Agropecuaria, Hurlingham, Argentina.

Chapman Kent, BioDiscovery Institute at the University of North Texas, Denton, USA.

Claverol Stéphane, CGFB, Bordeaux

Dörmann Peter, Institut für Molekulare Physiologie und Biotechnologie der Pflanzen Universitat Bonn, Allemagne.

Dyer John, US Arid-Land Agricultural Research Center, Maricopa, USA.

German-Retana Sylvie, UMR1332INRA, Bordeaux

Mullen Robert, Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.

Ngando-Ebongue Georges, IRAD-CEREPAH, Douala, Cameroun.

Rothan Christophe, UMR1332INRA, Bordeaux

Wenden Bénédicte , UMR1332INRA, Bordeaux

Former members:

Tuphile Karine, TCN 2015-2019

Nacir Houda, PhD 2011-2014 Combes-Soia Lucie, M2 2013 Esnay Nicolas, M2 2014 Bolik  Stéphanie, M2 2018 Bastien Guy, M2 2022

Bourdeau Pauline  M2 2020